Superheroes in Taq enzyme

It has been more than 30 years since Kary Mullis proposed the method of PCR in 1983 PCR has developed exponentially in the laboratory, bringing revolutionary progress to molecular biology, and the revolution continues to break out without any sign of slowing down With the application of PCR becoming more and more diversified, it means that researchers' demand for Taq enzyme function is becoming more and more diversified, and the era of "enzyme" taking the world is gone At present, the market of molecular biological reagents is basically wild-type DNA polymerases With the improvement of experimental requirements of customers and the complexity of biological samples, the limitations of wild-type DNA polymerases have gradually emerged in many experiments, such as low amplification efficiency, poor specificity, poor amplification results of samples with high at or GC content, interference of some inhibitors in samples or PCR system to the reaction, etc Kapa biosystems' proprietary direct molecular evolution platform is a highly customizable protein engineering technology, which can generate and screen a large number of enzyme mutants After several rounds of mutation, screening and amplification, the wild-type DNA polymerase gene finally obtains the required excellent characteristics They are "superheroes" in Taq enzyme For the template containing common PCR reaction inhibitors such as fecal samples, the robust synthesis ability of kapa2g robot DNA polymerase can obtain more stable results ·If there is a high demand for PCR speed, the extension speed of kapa2g fast DNA polymerase is as high as 1s / KB, and it has the characteristics of high sensitivity and high specificity Compared with traditional PCR reaction, it can save about 75% time ·If the sensitivity and speed of qPCR experiment are required, Taq enzyme in Kapa SYBR fast qPCR kits can improve the tolerance to fluorescent dye, and greatly improve the signal strength and response effectiveness Let's see the real strength of these superheroes! [as fast as lightning] kapa2g fast DNA polymerase is a second-generation engineering enzyme modified by wild-type Taq enzyme, which has higher synthesis ability and speed √ faster reaction: the PCR reaction time is shortened by 75% √ stronger synthesis: the extension time is shortened to 1 sec / KB √ more compatible: compatible with a variety of experimental samples, even samples rich in at - and GC - √ higher sensitivity and yield kapa2g rapid DNA polymerase application program: rapid identification of mouse genotype kapa2g rapid DNA polymerase can amplify 1KB per second at an extraordinary speed, which can be used in various research fields, such as rapid sample extraction + fastest kapa2g for rapid identification of mouse genotype DNA polymerase, from sample to result, can easily identify mouse genotype in one hour! Kapa2g rapid DNA polymerase assay by continuous monitoring of dNTP incorporation by fluorescence · stop flow DNA polymerase assay is the fastest PCR in the market at present! ·Kapa2g fast DNA polymerase with the near instantaneous heating and cooling realized by hardware, completed 30 cycles of PCR reaction in three minutes, unimaginably fast! [strong and visible] after years of experiments, you have deeply realized that experiments can't be perfect just like life: · after a lot of efforts, DNA templates still can't completely remove the inhibitory components · template sequence GC or at content is too high, you can't control · multiple DNA templates from different sources and different GC content can't use the same PCR scheme But just like life needs to bow down and work hard, experiments also need to make unremitting efforts Fortunately, there are strong kapa2g robot DNA polymerases to help you share and overcome the imperfections of these PCR experimental conditions Kapa2g robot DNA polymerase is a second-generation engineering enzyme based on the evolution of wild-type Taq enzyme, which is used to solve the amplification problem of wide-ranging amplification subtypes (rich in GC or at) It is suitable for challenging PCR applications and amplification of difficult samples, significantly reducing the high consumption caused by trying a variety of enzymes and the time wasted by constantly optimizing the amplification program Strong synthesis ability: resistant to the inhibitory components in PCR system, compatible with a variety of experimental samples Optimized crude samples, FFPE samples, at - and GC - rich samples, colony PCR reaction √ extension speed is faster than wild-type Taq enzyme: the extension time is shortened to 15-30 sec / KB, which helps the experimenter obtain the results faster √ higher sensitivity and Yield: especially suitable for experiments with limited sample size or low template content Click here to learn more about Kapa products If you want to do good things, you must first use the tools The same is true of scientific research Good tools can help the experiment get twice the result with half the effort, simplify the complexity and seize the time! 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