Summary: Take stock of the 2020 single-cell sequencing study - Tumor.

Introduction: single cell sequencing technology has been more and more used in basic scientific research and clinical research since it was published in 2009 and named as the annual technology by nature methods in 2013.single cell sequencing studies genome and transcriptome from a single cell level. By amplifying the whole genome or transcriptome, high-throughput sequencing can reveal the gene structure and gene expression status of a single cell, and reflect the heterogeneity between cells. Compared with population cell sequencing, it is more suitable for the study of a small number of special samples, the analysis of heterogeneous groups and the same time Or searching for genome changes that occur in mutual exclusion.single cell sequencing has important applications in tumor, developmental biology, clinical diagnosis, immunology, microbiology, neuroscience, large-scale cell map construction, cell subgroup Refinement & amp; rare cell type identification, stem cell development and differentiation, and so on.application fields of single cell technology source: flint creation at present, the most common application of single cell sequencing is in tumor research.the following is a summary of relevant studies in 2020: on June 25, 2020, the new DNA sequencing technology may help to reveal the genetic diversity of cancer tumors. Researchers at the University of Southern California (USC) used a single cell sequencing method based on microfluidic droplets, Sequencing of 500 single cell genomes revealed genetic diversity previously hidden in well studied melanoma cell lines.this study, published in nature communication biology, demonstrates the ability of single cell sequencing to reveal the possible evolutionary trajectory of cancer cells.doi: 10.1038/s42003-020-1044-82020.6.2 researchers identify moving targets in small cell lung cancer. In cancer cell, scientists at the University of Oregon outline new findings about the origin of small cell lung cancer subtypes, paving the way for new foundations for the study of the disease.they developed a new assay that allowed the team to track individual cells in tumor samples and observe their changes over time.this finding may also help to understand other cancers known to have subtypes, such as breast cancer or glioblastoma.the team speculated that other cancer subtypes may also represent the stage of tumor evolution.doi: 10.1016/j.ccell.2020.05.001, 2020.4.27 research found that immune system changes occur in the early development of multiple myeloma. A study published in nature cancer by Boston Dana Farber Cancer Institute, MIT and Harvard University is a step towards more personalized treatment of multiple myeloma.this new study represents the first application of single cell RNA sequencing.doi: 10.1038/s43018-020-0053-32020.4.17 single cell division error may trigger a series of mutations with cancer characteristics. A group of researchers from several institutions in the United States and the United Kingdom found that a single error in the process of cell division can trigger a series of mutation events, resulting in many features in the cancer genome.in a paper published in the journal Science, the team described the use of live cell imaging and single cell genome sequencing to study the effects of single cell mutation events on downstream cell development.this new study shows that a single error in cell division can lead to a series of mutations, resulting in the complexity seen in many cancer genomes.overall, this finding identifies a number of mechanisms that can explain the great potential of a single unrepaired DNA break to destroy genomic integrity.this discovery provides insights into the etiology of cancer and may promote new research to prevent mutations associated with chromobacillosis. DOI：10.1126 / science.aba07122020 . 1.29 single cell sequencing of CLL treatment: shared gene scheme, personalized implementation of patients by the research team led by the molecular medicine research center of the Austrian Academy of Sciences (CeMM), using single cell sequencing and epigenetic analysis methods to conduct a detailed study on the response to targeted leukemia treatment. the paper was published in the journal Nature communications. they combined immunophenotypic analysis, single cell transcriptome analysis (scrna SEQ) and chromatin localization (ATAC SEQ) to jointly monitor the activity, regulation and expression of chronic lymphocytic leukemia (CLL) cells and other cell types of the immune system. a precise molecular program was found in CLL patients who started using the targeted anticancer drug irutinib. although the program is shared by all patients, its execution speed varies greatly. the results help to develop personalized strategies for managing CLL as a chronic disease of the elderly. DOI：10.1038 / S41467-019-14081-6 an advertisement was inserted: CO sponsored by Shanghai Pudong New Area Biological Industry Association, Shanghai Zhangjiang biomedical Base Development Co., Ltd., gene detection technology branch of China Medical and Biological Technology Association, genetic diagnosis branch of China genetic society, Shanghai Genetics Society, Yangtze River Delta integrated gene detection alliance and translational medicine network Clinical application Salon of cell sequencing technology time: 13:30-17:30 PM, July 9, 2020 Venue: Zhangjiang · China Pharmaceutical Valley Biomedical Innovation exchange center (first floor, No. 781 Cailun Road, Pudong New Area, Shanghai) Click to read the original text and sign up for participation! Recommended reading: fight the epidemic situation, translational medicine network content team series report: [Nature] Blockbuster: a single designed DNA drug treatment is expected to completely cure Parkinson's disease! 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