Summary: Counting the 2020 Single Cell Sequencing Study - Oncology

Single-cell sequencing has important applications in the fields of tumor, developmental biology, clinicaldiagnosis,immunology, microbiology, neuroscience, large-scale cell mapping construction, cell subgroup refinement and rare cell type identification,stem celldevelopment and differentiation, and is the focus of research at presentSingle-cell technology applicationsSource: Firestone CreationCurrently, the most common single-cell sequencing application is in tumor researchThe following is a summary of the 2020 study:2020.6.25new DNA sequencing techniques may help reveal the genetic diversity of cancer tumors
researchers at the University of Southern California (USC) used a single cell sequencing method based on microfluidic droplets, while sequencing the genomes of nearly 1,500 single cells, revealing genetic diversity in previously hidden melanoma cell lines that have been well studiedThe study, published in Nature Communication Biology, demonstrates the ability of single-cell sequencing to reveal the evolutionary trajectory of cancer cells DOI: 10.1038 / s42003-020-1044-8 2020.62 researchers determine the movement target sized lung cancer in the journal Cancer Cells, university of Oregon scientists outlined new findings on the origin of subtypes of small cell lung cancer, paving the way for new foundations for studying the disease They developed a new method of measurement that allowed the team to track individual cells in tumor samples and observe how they changeover over time The findings may also help to understand other cancers known to have subtypes, such as breast cancer or glioblastoma The team speculates that subtypes of other cancers may also represent the stage of tumor evolution DOI: 10.1016 / j.ccell.2020.001 2020.4.27 study found that immune system changes occur in the early development of multiple myeloma a study published in Natural Cancer, Boston, MIT and Harvard University, is a step toward more personalized treatment of multiple myeloma The new study represents the first application of single-cell RNA sequencing DOI: 10.1038 / s43018-020-020-0053-3 2020.4.17 a single cell division error may trigger a series of cancer-like mutations
a group of researchers in several u.S and U.K institutions found that a mistake in cell division triggers a series of mutation events that can produce many of the characteristics of the cancer genome In a paper published in the journal Science, the team describes the use of live cell imaging and single-cell genome sequencing to study the effects of single-cell mutation events on downstream cell development The new study suggests that a single error in cell division can lead to a series of mutations that create the complexity seen in many cancer genomes overall, the discovery identifies a number of mechanisms that explain the enormous potential of a single unrepaired DNA fracture to undermine genomic integrity The findings provide some insight into the etiology of cancer and may lead to new research to prevent mutations associated with coloring bacteria DOI: 10.1126 / science.aba0712 2020.1.29 single-cell sequencing CLL therapy: Shared gene programs, patient-specific execution a research team led by the Center for Molecular Medicine of the Austrian Academy of Sciences (CeMM), a detailed study of the response to targeted leukemia treatment using single-cell sequencing and epigenetic analysis methods The paper was published in the journal Nature Communications They combine immunotype analysis, single-cell transcription group analysis (scRNA-seq) and chromatin localization (ATAC-seq) to monitor the activity, regulation, and expression of chronic lymphocytic leukemia (CLL) cells and other cell types of the immune system Precise molecular procedures were found in CLL patients who began using the targeted anti-cancer drug Ilutinib Although the procedure is shared by all patients, the speed at which it is performed varies widely The results of the study help to develop a personalized strategy for the management of CLL as a chronic disease of the elderly DOI: 10.1038 / s41467-019-14081-6 Source: