-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
| Successfully developed a green bio-manufacturing chassis cell high-efficiency editing tool |
Zymomonas mobilis repairs damage to genomic DNA and movable elements caused by the CRISPR-Cas system
.
Photo courtesy of Central China Agricultural University Zymomonas mobilis repairs damage to genomic DNA and movable elements caused by the CRISPR-Cas system
Zymomonas mobilis( Zymomonas mobilis ) is a bacteria that can directly use the ED pathway to metabolize sugars to produce ethanol under anaerobic conditions.
It has the characteristics of high ethanol fermentation rate, good ethanol tolerance, and biosafety.
The application has great application value
.
The research team found that the genomic DNA damage caused by the CRISPR-Cas system can be efficiently repaired by the micro-homology-mediated joining pathway (MMEJ), and part of the DNA is missing
.
The MMEJ system has a very low efficiency in repairing the damage of moving elements caused by CRISPR-Cas, which represents a recognition mode that distinguishes itself from foreign DNA after cutting
Journal of Genetics and Genomics
On this basis, the research group used the endogenous CRISPR-Cas system and the MMEJ approach to develop a tool suitable for rapid genome editing and essential gene identification of Z.
mobilis
.
The tool only needs to construct a self-targeting plasmid with mini-CRISPR to achieve the knockout of target genes and long fragments
Biotechnology for Biofuels
Pediococcus lactis is an important probiotic in the feed and medicine catalogs.
It is widely used in production practices and clinical treatments such as lactic acid and feed fermentation and immune regulation
.
The research team isolated a strain of Pediococcus lactis with good production performance and strong probiotic properties in the early stage, and found that its genome encodes the CRISPR-Cas9 system
Although the cas9 gene has a nonsense mutation, research by the research team has shown that it can still perform the nucleic acid cleavage function
.
Based on the endogenous CRISPR-Cas9 system, the research team has established efficient genetic manipulation tools to achieve efficient gene knockout, point mutation and integration
Applied and Environmental Microbiology
Related paper information:
https://doi.
https://doi.
https://doi.
https://doi.
org/10.
1128/AEM.
00948-21
