Study on the effect and mechanism of Trifolium extract on the apoptosis of human lung cancer H1299 cells

Recently, researchers from the Department of oncology, Tongde Hospital Affiliated to Zhejiang University of traditional Chinese Medicine published a paper to explore the effect and mechanism of Trifolium extract on the proliferation inhibition and apoptosis of human lung cancer H1299 cells The results showed that the extract of Trifolium could inhibit the proliferation and promote the apoptosis of human lung cancer H1299 cells, and its mechanism might be related to the activation of caspase protein expression This article was published in the 11th issue of Chinese Journal of integrated traditional and Western medicine in 2014 Cell counting kit-8 (CCK-8) was used to detect the inhibitory effect of Trifolium extract on the proliferation of H1299 cells; inverted microscope was used to observe the effect of Trifolium extract on the morphology of H1299 cells; fluorescence microscope was used to observe the apoptotic morphological changes of cells treated by Trifolium extract after Hoechst 33258 staining; flow cytometry was used to detect the apoptotic rate; Western blotting method was used The expression of pro-caspase-3,9, cle-caspase-3,9, poly ADP ribose polymer (PARP) was detected by blot Compared with the control group, the inhibition rate of 0.5, 1, 5, 10 mg / ml trefoil extract on H1299 cells increased (P < 0.05, P < 0.01); at the same time, the longer the time, the higher the inhibition rate (P < 0.01) Under the inverted microscope, the morphology of the cells changed obviously and the number of cells decreased Under the fluorescent microscope, the nuclear concentration and the formation of apoptotic bodies were observed The results of flow cytometry showed that the apoptotic rate showed a significant dose-response relationship The results of Western blot showed that: compared with the control group, the concentration of 5 and 10 mg / ml of trefoil extract significantly inhibited the expression of pro-caspase-3, 9 and PARP protein in H1299 cells, increased the expression of cle-caspase-3, 9 and cle-parp protein, the difference was statistically significant (P < 0.05).