Study on the curative effect of "Lifukang" on pullorum and fowl cholera

Abstract: in this experiment, the bacteriostatic test of rifukang on Salmonella and Pasteurella and the treatment test on artificially infected chicken showed that rifukang tablet had good therapeutic effect on pullorum disease and fowl cholera, and the death rate of the treatment group was significantly lower than that of the control group (P < 0.01) It is better than norfloxacin It is an ideal non-toxic and no side effects new Chinese herbal medicine preparation Key words: the curative effect of Lifukang tablet on chicken diarrhea and fowl cholera; chicken diarrhea and fowl cholera are several common diseases and frequently occurring diseases that seriously affect the development of poultry industry in China Every year, it causes great losses to the poultry industry of our country At present, although there are many control methods such as vaccine and chemical antibacterial agent feeding, the effect is not ideal There is no effective vaccine for pullorum, and the pathogen is resistant to antibiotics Although there are many kinds of vaccine for avian cholera, there are some problems such as short immune period and poor effect, which are difficult to be popularized The application of antibiotics also has the problems of resistance and drug residues Therefore, it is urgent to find a new control method Traditional Chinese medicine is a great treasure house In recent years, the application of Chinese herbal medicine in the prevention and treatment of poultry diseases has developed a Chinese herbal medicine preparation "Lifukang" tablet, which has no drug resistance, no toxic side effects, no residue and obvious effect, and has achieved remarkable results The report is as follows: 1 Materials and methods 1.1 experimental drugs Lifukang tablet, a traditional Chinese medicine preparation, was developed by the animal medicine research center of Chongqing Pig Breeding Research Institute and produced by the animal medicine factory with a specification of 0.3g × 24 tablets × 2 plates and a batch number of 20020803 The control drug, 2.5% norfloxacin premix, was prepared by the animal drug research center of Chongqing Academy of pig breeding, with a specification of 10G × 50 bags 1.2 test animals and groups: 320 2-week-old isa commercial cocks were purchased in the market During the test, they were randomly divided into 8 groups, 4 groups for each disease, 40 for each group, and kept in cages for future use 1.3 Salmonella c79-10 and Pasteurella multocida standard strain c48-1 were introduced from China Veterinary Medicine Supervision Institute and then stored by the veterinary research center of our hospital after rejuvenation 1.4 for artificial disease, the preserved strains were inoculated with special culture medium respectively, and then the typical colonies were cultured in broth for 24 hours as the virulent bacteria The amount of attack was as follows: 1.0ml of Salmonella solution (about 2500 bacteria / 1.0ml) was injected into the abdominal cavity of each chicken in the pullorum group; 2.0ml of Pasteurella solution (about 2000 bacteria / 1.0ml) was injected under the skin of each chicken in the cholera group After inoculation, observe the spirit, feeding, drinking water, feces, respiration and other clinical conditions of the experimental chickens in each group; carry out pathological dissection on the dead chickens, and separate and culture the bacteria from the heart blood and viscera 1.5 in the treatment trial, 36-48h after the attack, the test chickens showed typical symptoms, and then they were given medicine according to table 1 for 3 consecutive days and 14 days in the trial period The chickens in each group were regularly observed in terms of food intake, drinking water, mental status, clinical symptoms, and death records, and the dead chickens were dissected and bacteria separated 1.6 mortality: the percentage of chickens with typical symptoms and pathological changes in the experimental group Apparent efficiency: at the end of the experiment, the percentage of live chickens in each group in the experiment group Cure rate: the percentage of chickens in the experimental group whose clinical symptoms disappeared completely after treatment Data statistical processing: routine statistical method (x test) was used to test the mortality and cure rate of each group See Table 1 for infection and medication of two kinds of experimental chickens Table 1 Experimental chicken infection and medication (the two disease groups are the same) (omitted) 1.7 "rifukang" bacteriostatic test on Salmonella pullorum and Pasteurella multocida Rifukang decoction was used to make the filtrate into 1:1 solution, and the plate hole digging method was used to carry out the bacteriostatic test to observe the bacteriostatic effect 1.8 promotion and application: promote the application of "rifukang" tablet in and out of the city, collect application data and effect response 2 Results and discussion 2.1 The curative effect on pullorum disease in chickens 2.1.1 artificial infection and control test results the main clinical manifestations of chickens after attacking the virus are depression, fluffy feathers, loose stool in the shape of white paste, dyspnea, emaciated physique, loss of appetite and death from exhaustion In the control group, the pathological changes were typical In laboratory diagnosis, the dead chicken liver and spleen were inoculated on the ordinary plate agar medium and cultured at 37 ℃ for 24 hours to produce smooth, moist, bulging and translucent colonies On the SS plate, the colonies were five colored, transparent and smooth, with a core, which was identified as Salmonella by biochemistry Within two weeks after administration, the mortality and cure rate of each group are shown in Table 2 Table 2: the control test results of "rifukang" on chicken diarrhea caused by artificial infection (omitted) 2.1.2 test shows that the mortality of rifukang tablet group and norfloxacin group is significantly lower than that of the control group, and the cure rate of rifukang tablet group is slightly higher than that of norfloxacin group 2.2 the therapeutic effect of "rifukang" on Pasteurellosis (fowl cholera) 2.2.1 the results of artificial infection and control test 2.2.1 the main clinical manifestations of the experimental chickens after the attack of virus are mental upset, fluffy feathers, acute diarrhea, dyspnea, rhinorrhea and loss of appetite Most of them died 1-2 days after the attack In the control group, the pathological changes were typical In laboratory diagnosis, the dead chicken liver, spleen and other disease materials were inoculated on blood agar medium, cultured at 37 ℃ for 24 hours, and the small colony with gray white and dewdrop shape grew; it was identified as Pasteurella The administration group began to take the medicine when the first onset of depression occurred Within two weeks after the administration, the mortality and cure rate of each group are shown in Table 3 Table 3 the results of the control test of rifukang (omitted) 2.2.2 Table 3 show that the mortality of rifukang tablet group and norfloxacin group is significantly lower than that of the control group, and the cure rate of rifukang tablet is slightly higher than that of norfloxacin group 2.3 safety test: 30 7-day-old chickens were fed with 10 times of the therapeutic amount of "Lifukang" powder for 15 days without any adverse reactions, indicating good safety 2.4 expanded test and application: 3680 chickens with dysentery, 3389 chickens, 92.09%; 4325 chickens with cholera, 4049 chickens, 93.62%; 1869 chickens with colibacillosis (peritonitis, ovarian cyst, pericarditis, etc.), 1798 chickens, 96.20% Since it was approved for pilot production and put on the market, 20 million copies have been popularized and applied, and the general response effect of users is obvious The products are exported to more than ten provinces and cities in China, and remarkable social and economic benefits have been obtained The experiment proved that the traditional Chinese medicine "rifukang" has a significant effect on the main bacterial diseases of chickens 3 Conclusion 1 The results of this experiment showed that the Chinese medicine Lifukang tablet had a good therapeutic effect on pullorum disease and fowl cholera The mortality of the treatment group was significantly lower than that of the control group (P < 0.01) It is better than norfloxacin 3.2 "rifukang", a pure traditional Chinese medicine preparation, can not only achieve the treatment purpose, but also be added in the feed for a long time to prevent the occurrence of chicken dysentery, avian cholera and colibacillosis It is an ideal non-toxic and non side effect new Chinese herbal medicine preparation, which can replace some antibiotics in the prevention and treatment of avian bacterial diseases.