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Sterilization and preservation of tissue cultured culture

 Last Update: 2021-03-07
 Source: Internet
 Author: User

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base 20

tissue culture

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culture
should be sterilized immediately after the preparation of the base.
medium
usually sterilized in a steam-pressure sterilization pot for 20 minutes at
120 degrees C. If there is no steam-pressure sterilization pot, can also be sterilized by intermittent sterilization, the medium is boiled for 10 minutes, 24 hours after boiling for 20 minutes, so that three consecutive sterilization, you can achieve the goal of complete sterilization.The sterilized culture base should be stored at
10 degrees C, especially those containing growth-regulating substances, and should be stored at a low temperature of 4 to 5 degrees C. A culture containing pyridine or erythromycin should be used within one week of preparation, and other cultures should not exceed one month at most. In most cases, it should be used up within two weeks of disinfection.

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