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Potato virus disease is a prominent problem hindering the development of potato production around the world, over the years experts use
organizations
culture
for stem tip detoxification, showing that once solved the harm caused by viral disease to potato production, but because of the application of this technology to produce detoxifying original species high cost, slow speed, labor intensity, flower work, has always been in a situation. To this end, experts used soilless cultivation methods for the factory production of detoxified potato seed success. This method greatly improves the reproduction of detoxifying potatoes, reduces the cost, by the vast number of farmers and experts praise. , technical advantages
(i) production advantages in soilless cultivation under the conditions of the production of detoxifying seed potatoes are not limited by the time season, all year round can be produced in greenhouses. Moisture, light, temperature can be manually controlled, different physiological characteristics of different varieties, pharmaceutical preparation can be carried out to maximize their physiological
biochemic
requirements. Coupled with good breathability, so that the root system grows fast and developed, plants lush, leafy green, root system increased by 35%. 3-5d earlier than the production of mesh shed live trees, no slow seedling period, strong absorption capacity, increased growth potential energy, than the net room early potato 7-8d or so. As a result of growing in the warm room, the occurrence of late epidemic disease was controlled, the growth time was prolonged and the yield was increased.(ii) cost and efficiency advantages of the use of soilless cultivation factory production of detoxified seed potato original species, covers a small area, easy to manage, when the seed potato in about 25g, at any time can be deleted from the culture box,
string temperature
constant humidity preservation for use. Due to different varieties, each plant can be detoxifying small potato 80-140 or so, equivalent to 10-12 plants in the shed, saving about 40 yuan in the cultivation of weed mu, production efficiency for the shed 8-14 times, the cost of small potatoes decreased by more than 40%, than the shed transplanted production of the original labor intensity reduced by more than half, easy to promote large areas of detoxifying potato cultivation and production, improve yield, increase economic income per mu.II, the application mode
(i) site and facilities installation according to the actual situation of the unit, in the sun to establish a glass greenhouse, in winter with daylights to heat. Conditional can also be built a modern automatic air-conditioned greenhouse. Automatic photosensitometers, hygrometers,
thermometers
are installed in the room to monitor the environment of growing plants, and when the light temperature is high, a sunshade can be added, water splashed to cool down or vent ventilation can be opened. Hydroponic box from the ground 60-70cm is good, easy to work, box width 60cm, high 45cm, adjustable length, the inner layer of the box with insulation plate with black plastic film wrapped composition, cover plate with a black plastic film insulation plate. In the cover plate into a plant line distance of 20cm×20cm uniform small hole, each hole put a plant, MS nutrient liquid with non-toxic plastic buckets buried half in the ground, to maintain the same temperature as the ground, for the pipe with non-toxic plastic pipe, 1m apart from the pipe to put a small hole in the nozzle, pipe in the water tank around a week. Put a small hole in the bottom of the box that is filled with nutrients to connect the liquid back into the barrel, nutrition liquid with liquid pump every 3 minutes time spray 3 seconds.(ii) The cultivation and planting of seedlings
1. Growth point culture stage: the potato block with NaCl disinfection, distilled water rinsed 2 times, and then 70% ethanol disinfection, with 100x10-6 erythromycin treatment to make the potato block grow small buds, and then the buds with the above method to disinfect again. Place the disinfected buds under 40 times
microscope
and use an anatomy needle to place the growth point under the sterilized
test tube medium
and cover the paper cap in the culture room for culture. 2. Seedling stage: small seedlings cultivated in the culture room, placed in the warm room, in the light is strong, the temperature is about 25 degrees C in the area of seedling 5-7d, so that it becomes coarse and strong. 3. Planting period: put the well-exercised seedlings on the cover plate that has been punched well, preferably on cloudy days or after 3 p.m. every day, before planting the greenhouse with Formarin 100 times liquid spray or smoke steaming 2.25kg/100m2 and spray water to make it saturated. Then the seedlings are removed and inserted under the board, under the board to have buds, the board left 2-3 leaves, seedlings planted after 5-7d for the transition stage, the first 2d to use water spray, 3d after the use of 50% concentration of nutrient liquid spray, after the nutritional growth period with nutrient liquid spray. During this period, the number of hours of sun requires 13-15 hours / day, the minimum can not be less than 2000 lux, the temperature of the nutrient solution is maintained at 18 degrees C - 20 degrees C, humidity 70%-80% is appropriate.