Sn2019: neural stem cells with a life span of up to 90 days have been identified for the first time in living mice

October 24, 2019 / Biovalley BIOON / - -- in an unofficial study, Gregor Pilz, a neuroscientist at the University of Zurich in Switzerland, observed that neural stem cells turned into neurons in the brain of young living mice, a feat not long ago considered impossible His latest experiments show that there are another group of self renewing neural stem cells in the mouse brain, some of which have a longer life span than the first ones he recorded Pilz discussed the results at a meeting of the society for neuroscience in Chicago on October 22, 2019 If confirmed, it may help to reveal the changes of neurogenesis - the generation of new neurons - in the process of animal aging Newborn neurons (yellow, orange and red) are located near neural stem cells (green) The neurons grew in the hippocampus of adult mice over a two-month period, pictured in the University of Zurich Past studies have identified different neural stem cell populations, but it has been difficult to track their changes over time in the brain of living mice The part of the hippocampus that contains these stem cells is called the dentate gyrus, which is located deep in the brain, so scientists have to remove the tissue above it to observe the neural stem cells in living mice, and they need tracers to mark the specific stem cells they want to observe In a paper published in the journal Science in 2018 (Science, 2019, DOI: 10.1126 / science Aao5056), Pilz and his colleagues, as a postdoctoral researcher in the Sebastian jessberger Laboratory of the University of Zurich, described how they monitored cell division in neural stem cells labeled with green fluorescent protein (GFP), which is expressed under the drive of ascl1 ASCL 1 protein plays a role in neural differentiation According to reports, these neural stem cells experienced "rapid neural burst", which made them produce 5 neurons on average in two months or less, and then disappear in the mouse brain In their latest experiment, Pilz and his colleagues tracked neural stem cells that lived relatively long After being labeled with GFP, which is driven by Gli1, these neural stem cells generate five neurons over a 90 day period, and then disappear Gli1 protein also plays a role in neurogenesis Pilz told the scientist that the results showed a "diversity" of neural stem cells in the dentate gyrus His team's research has also begun to reveal details about molecular and cellular differences that determine how long neural stem cells can survive once they begin to divide, and may also provide clues to the cellular mechanisms that lead to a decline in neurogenesis during aging Pilz pointed out that his team studied adolescent mice, not adult mice, in these latest experiments, but said that this technology has enabled the team to track the fate of different neural stem cell populations that it has been tracking during the mouse's transition from adolescence to adulthood He said the experiments may reveal whether aging has an adverse effect on these cell lineages His team can now also see what happens to these neural stem cells and new neuronal populations when these mice are moving or socializing - both of which are related to increased neurogenesis in adult mice Pilz said the results may identify "physiological conditions that actually contribute to neurogenesis, activate these neural stem cells, or support neuronal survival." (BIOON Com) reference: 1 Adult hippocamporal neurogenesis in humans and roads: new evidence and new perspectives - Juan song https://www.abstractsonline.com/pp8/ ා! / 7883 / presentation / 3112 Long live neural stem cells identified in living rice https://www.the-scientist.com/news-opinion/long-lived-neural-stem-cells-identified-in-living-mice--66614 3.Gregor-Alexander Pilz, Sara Bottes, Marion Betizeau et al Live imaging of neurogenesis in the adult mouse hippocampus Science, 09 Feb 2018, 359(6376): 658-662, doi:10.1126/science.aao5056.