Shanghai Chengsi Resistance Dry Microbial Penetration Tester CSI-287 Technical Exchange and Analysis

Numerous examples of dry-state microbial penetration testers have shown that bacteria can penetrate shielding materials with dry organic or inorganic particles, such as bacterial-carrying dander or clean clothes, and bacteria-carrying particles penetrate through the storage period.
packaging material
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This instrument can be used to determine the resistance of materials to bacterial penetration on dry particles in the size range of human dander
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　1.
Test principle and technical specifications 1.
Test box: black inner wall, volume: 0.
5m3, built-in exhaust fan and lighting; 2.
Instrument air source: 0.
8MPa compressed air; 3.
Noise: about 90dB; 4.
Touch screen operation; 5 .
Instrument size: 1000mm´690mm´1390mm; 6.
Instrument weight: 85kg 7.
Power supply: 220V
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　　Configuration Checklist This instrument should be carefully unpacked and inspected
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Any damage or loss of the instrument, please contact our customer service department
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　　When you open the package, please check the following materials (subject to the actual packing list): 1.
1 host; 2.
8 sets of test boxes; 3.
1 power cord; 4.
1 copy of the manual 5.
Optional Accessories: 6.
Air storage tank 2.
Test standard Dry microbial penetration tester is used to test samples according to ISO 22612, YY/T 5056.
5 test standards
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　　3.
The composition and use of the instrument The instrument is completed in strict accordance with the standard drawings, and fully meets and can realize the test content required by the standard
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　　The instrument includes: (1) Pneumatic ball oscillator: The external air source passes through the pneumatic ball oscillator, and the vibration frequency is maintained at the target frequency through corresponding adjustment and correction
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It can generate 20,800 vibrations per minute with a force of 650N
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　　(2) Compressed air flowmeter: It is mainly used to measure the airflow that generates 20800 (347Hz) vibration frequencies per minute
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　　(3) Test container: The instrument has 6 stainless steel test containers with metal pistons on the top
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The metal piston can be inserted through the central hole on the cover to reach 10mm below the cover, and ensure that the test piece is not loose after insertion
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At the same time, each container bottom attachment has a slit into which a petri dish is inserted
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　　(4) Marble board: marble with an area of ​​400mm*400mm and a thickness of 10mm is used as the operating platform of the whole instrument
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　　(5) Fixing plate: a stainless steel plate with 6 holes, which is fixed on the stone plate with a jig, used to fix the installation of the test container (6) Rubber support: with 4 rubber supports, used to adjust the level and fix the instrument position to prevent movement
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Test 1.
Analysis of test steps: This test is carried out on the specimens fixed on a container respectively
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Among these containers, 5 containers with Bacillus subtilis talc f were used as the test group, and 1 container with uncontaminated talc f was added as a control
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Insert a petri dish at the bottom of each container close to below the test piece
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　　The device supporting the container is oscillated by a gas ball shaker, all the talc f penetrating the test piece falls on the petri dish, the petri dish is taken out and cultured
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　　The number of growing colonies was counted
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　　2.
Equipment debugging: Ensure that the dry-state microbial penetration tester is operational
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　　(1) Adjust the level According to the level, adjust the 4 rubber supports to make the instrument in a horizontal position
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　　(2) Adjust the frequency of the gas ball oscillator The vibration frequency of the gas ball oscillator is 20,800 times per minute by adjusting the gas ball oscillator
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　　3.
Sample preparation: (1) Cut 12 samples to be tested and the size of 12 samples is 200mm×200mm
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　　(2) Sterilization treatment The cut specimens and each test container are sterilized
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　　(3) Fixing the test container Fix the test container on the fixed plate through a rubber ring, so that it is closely connected with the fixed plate, and the bottom is against the stone plate
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　　(4) Talc f Prepare to prepare talc f containing AATCC 9372 Bacillus subtilis spores, and the concentration is preferably about 108 CFU/g, and the preparation process refers to YY/T 5056.
5-2009
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　　4.
Start the test
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　　Take out the test piece by aseptic method and put it on the mouth of each test container
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　　The lid is secured to the container by pushing down on the plunger so that the test piece is secured with a controlled degree of relaxation
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　　Remove the piston
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Add 0.
5g±0.
1g of talc f stained to the test piece through the piston port, and add unstained talc f to the sixth container as a control
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Cover the piston port with film
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　　Put a small plastic bag over each container
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　　Insert the uncovered petri dish through the slit in the bottom of each container
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Close the slit with adhesive tape
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　　Vibrate for 30min at a vibration frequency of 20,800 times per minute
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　　Remove plastic bags and adhesive tape
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　　Insert the lid of the petri dish through the slit, remove the petri dish and incubate at 35°C for 24h
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　　Colonies formed were counted
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A valid sample should be a control dish with a 0 reading
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Otherwise, there is foreign pollution, and the test should be terminated
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　　Repeat the above experimental steps
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　　At least 2 groups were tested, and the arithmetic mean of 10 valid results was calculated as the final result
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　　The end of the experiment When the experiment is over, make sure the data has been recorded, and adjust the pressure regulator knob on the panel to 0 scale
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Turn off the instrument air intake, turn on the exhaust fan and lighting, and clean the surfaces of each sample cell
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　　5.
Maintenance Keep the instrument dry In order to ensure the normal use of the instrument and prolong its service life, please keep the instrument dry
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And use it in a relatively dry environment to avoid water droplets splashing or condensation, so as not to damage non-water-resistant devices
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If the instrument will not be used for a long time, please disconnect the power supply and the gas source
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　　Component damage The internal start-up oscillator of the instrument is a vulnerable component, and when it reaches its service life, the accuracy of the oscillation frequency will decrease
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It is recommended to replace the new parts at this time
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　　At the same time, if the marble plate is damaged, please replace the accessories of the same specification in time
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　　Microbial safety In order to prevent contamination of the test personnel and the test environment, the test personnel need to have an operational basis for microbial culture
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Follow laboratory practices carefully
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