Several treatment recommendations after the appearance of glial worm in cell culture.

Biotechnology Channel News: "Blastin" can live in animal cells, can also survive in the medium, relying on the nutrition in the cell and medium for a living, and passed down with the cells.
can be seen that "black gum worm" is a kind of heterogenous creatures.
what if we contaminate after the appearance of glial worms in cell culture? Here are a few suggestions for dealing with it, let's take a look at it! 1, a better serum, of course, it is best to import fetal cow serum (domestic serum is too dirty), that is, the price is outrageous, economic conditions do not allow, can be used to import new cattle serum instead.
suggest that if a hyclone or GIBCO serum is used, it is not necessary to inactivation, which can effectively reduce the formation of "small black spots".
general serum is not to be inactivation treatment, why? Because the properly treated thermal inactivation serum is not needed for most cells.
the treated serum has only a slight boost to cell growth, or has no effect at all, and is often caused by high temperature treatment affecting the quality of the serum, resulting in a decrease in cell growth rate.
And after the hot inactivated serum, the formation of sediment will be significantly increased, these sediments in the inverted microscope observation, such as "small black spots", often let researchers mistakenly think that the serum is contaminated, and put the serum in the environment of 37 degrees C, will make this sediment more, so that researchers mistakenly believe that the microbial division and expansion.
recommended for thermal inactivation unless you are doing immunological research or growing stem cells, insect cells, and smooth muscle cells.
so when not doing immunological research or cultured stem cells, insect cells, and smooth muscle cell culture, the serum tries to be inactivated without heat.
2, if it is wall cells, can be washed with sterile PBS several times, can be alleviated to a certain extent.
if it is suspended, it is not very good to deal with pull, you can add a little bit of nourishing cells to it.
nourishing cells are effective against newly resuscitation cells with glial worms! There is also the experimental environment to pay attention to good, to maintain the cleanliness of the entire environment between cells.
if the cells are not very precious, they can be washed with bergamide, sulfonamide, tetrone, Benir, and also recommended cinnamycin 500ug/ml.
3, exchange imported disposable plastic culture bottles.
4. Stop resuscitation, stop cell culture, thoroughly disinfect all supplies used for cell culture, including culture bottles, methyls, straws, pancreases, incubators, ultra-clean stations, spaces, and all the things you can think of and all that is relevant to cell culture.
week after the accident, resuscitat the cells that were frozen before the contamination, and pay attention to the contamination of your white coat sleeve.
you may think it's too much, but it can take a week to save two months, and there's other effort and money you put into finding sources of pollution and removing it.
5, before the change of fluid to add physiological saline and gently beat, rinse clean and then add culture fluid, adhere to day-to-day washing, pass-through with physiological saline and centrifugation once again, vaccination density slightly larger, after a period of time, the insect will be greatly reduced, cell growth will not have a big impact.
It is best to re-match everything; if you really want to rescue, focus on breakthrough, leave a few bottles of contaminated not very serious cell rescue, the rest abandoned 6, if there are other available cells, then the contaminated cells are best thrown away; if not, you can try antibiotics, the most reliable is the bacterial culture drug sensitivity, according to drug sensitivity results to choose antibiotics, of course, can not affect the state of cells.
7, there are materials called minocycline has a certain role, can be combined with the use of fluid exchange.
because glial worm has not clearly identified what kind of organisms, so the above treatment method is not necessarily correct, can be considered for use.
8, someone in order to find a way to kill the "black glue worm", did an experiment, he took the "black glue worm" contaminated six-hole plate, each hole has 2 ml of culture solution, to the contaminated hole to add 0.5 ml, 1 ml, 2 ml, 3 ml of the new Gel anti-liquid, side-by-side in the inverted microscope observation.
he concluded that the ratio of new gel to water or culture was 1:4 to kill the "black bug".
but the negative effects of the new Jairni on cells are too great, and it is best not to use this method for more precious cell culture.
In cell culture experiments, people encounteredgumworms are not all the same, there are like bacteria, like mycelium, like protobiotics, like fungi, and like cell fragments.
in the domestic laboratory equipment conditions are uneven, and the quality of the operation of the experimenters are not the same, the same phenomenon may also be somewhat different, when described the differences may become between large or small.
So many people see cells contaminated by bacteria, not easy to deal with, but the phenomenon described is similar to the spread of glial worms, it will be the reason for the failure of culture to the undetlowableglial contamination, or even that it found is a real glial worm.
more such things, the glial worm will be artificial variants, like everything has.
the existence of icing on the glial worm has yet to be developed scientifically.
But one thing is certain, most people found the " glue worm" is not the real unknownglue worm, but not very common bacteria, fungi, progeny and other microbial contamination.
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