Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors
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Last Update: 2020-12-03
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Source: Internet
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Author: User
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The dideoxy chain-termination method (
1
) involves enzymatic elongation of a oligonucleotide primer that is annealed to a single-stranded
DNA
template. Single-stranded DNA of bacteriophage M13 (
2
) or phagemid vectors (
3
) give the most consistently satisfactory sequencing data. However, denatured double-stranded plasmid DNA can also serve as a suitable template and can also yield several hundred nucleotides of sequence information per reaction.
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