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Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene

 Last Update: 2021-01-12
 Source: Internet
 Author: User

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Acetolactate synthase (ALS) is an enzyme in the biosynthetic pathway for branched-chain amino acids, and bispyribac-sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of ALS activity. However, it appears that a mutated form of rice
ALS
[
OsmALS
(W548L/S627I)] confers resistance to BS. We succeeded in using
OsmALS
with native
OsALS
promoter and terminator region for a selection marker of rice transformation. Because this selection marker cassette is originally from the rice endogenous genome, it can be expected to be publicly acceptable.

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