Selection and symptomation of antibodies against dengue virus

Title: Selection and Characterization of Anti-Dengue NS1 Single Domain Antibodies
Journal:
Lisa C. Shriver-Lake, Jinny L. Liu, Dan Zabetakis, Victor A. Sugiharto, Cheng-Rei Lee, Gabriel N. Defang, Shuenn-Jue L. Wu, George P. Anderson, Ellen R. Goldman
Published: 2018/12/27
Digital ID: 10.1038/s41598-018-35923-1
Original link:
Reliable detection and diagnosis of dengue virus (DENV) is of great significance for patient care and epidemiological control. A recent paper in Scientific Reports by Ellen R. Goldman of the Center for Biometric Science and Engineering at the U.S. Naval Research Laboratory looked at this issue.
First, the researchers inoculated a mixture of recombinant nonstructive protein 1 (NS1) antigens from four DENV serotypes into a llama, then built a monolithic antibody phage display immune bank, from which they selected antibodies that demonstrated specificity and affinity to DENV NS1. They then evaluated the binding affinity of these monolithic antibodies to NS1 from four serotypes and used them in sandwich-type detection methods to detect NS1.
researchers selected the best antibody pair, which has the best sensitivity to the four DENV NS1 antigens added to 50 percent of the human serum, and does not cross-react with NS1 from Zika virus, yellow fever virus, and tick-to-encephalitis virus, and is largely non-binding to NS1 from Japanese encephalitis virus and West Nile virus. These robust and stable recombinant binding molecules may be able to replace conventional antibodies for immunology testing in resource-limited areas.
summary: Reliable detection and diagnosis of dengue virus (DENV) is important for both patient care and control. Starting with a llama immunized with a mixture of recombinant nonstructural protein 1 (NS1) antigen from the four DENV serotypes, a phage display immune library of single domain antibodies was constructed and binders selected which exhibited specificity and affinity for DENV NS1. Each of these single domain antibodies was evaluated for its binding affinity to NS1 from the four serotypes, and incorporated into a sandwich format for NS1 detection. An optimal pair was chosen that provided the best combination of sensitivity for all four DENV NS1 antigens spiked into 50% human serum while showing no cross reactivity to NS1 from Zika virus, yellow fever virus, tick-borne encephalitis virus, and minimal binding to NS1 from Japanese encephalitis virus and West Nile virus. These rugged and robust recombinant binding molecules offer attractive alternatives to conventional antibodies for implementation into immunoassays destined for resource limited locals.
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(Source: Science.com)