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Plant scientists are actively working to successfully introduce valuable traits into plants and use them as bioreactors for "molecular grafting," ie, the production of medicinally useful molecules in living organisms that act as chemical factori.
To rewire the metabolic network, multiple genes must be expressed under different promoters (regions of DNA) that trigger the expression of specific gen.
To address these knowledge gaps, a research team from China used a systematic approach to screen promoter and terminator libraries and provisionally assessed their performance in a reporting ass.
The researchers used promoter sequences from a variety of plants and viruses, including Arabidopsis, maize, potato, rice, and retroviruses that infect plan.
Among the 13 terminator sequences, the Arabidopsis T_AtHsp12 terminator outperformed the commonly used T_35S and T_nos terminato.
To give strong support to their findings, they went on to provide a proof of concept in plants using the betaine protease synthesis pathw.
The results show that quantitatively characterized promoters can ensure optimal expression of gen.
