Scientists have overcome the problem of G protein conceded conductor signal transduction

The cross-cutting team, led by Xu Huaqiang, a researcher at the Shanghai Institute of Pharmaceutical Research of the Chinese Academy of Sciences, successfully analyzed the near-atomic resolution structure of the erythroid and inhibited G protein (Gi) complex using cryoelectral technology to overcome major scientific problems in the field of cell signal transducation. On June 14, the results of the study were published online
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GPCR is the largest class of cell cross-membrane signal transductive subject families and the most important drug targets, which transduct extracellular signals through a variety of effect proteins, such as conjunged downstream G proteins and deterring proteins. In a variety of GPCR effect proteins, inhibitory G protein selectively coupled GPCR, such as pythromylamphetamine and dopamine, to regulate mood, appetite, motivation, cognition and reward functions.
Xu Huaqiang team obtained a near-atomic resolution frozen electroscope structure of the cyanoid and Gi protein complexes in the study. For the first time, the structure shows the structural details of the interaction interface between GPCR and Gi protein, perfects people's understanding of the molecular mechanism of GPCR-Gi downstream transduction selectivity, and provides a structural basis for the design of high-efficiency, low-toxic GPCR targeted drugs.
this work is another important breakthrough for Xu Huaqiang and his partners in the field of GPCR research. In 2015, it used X-ray free electron laser technology to solve major scientific challenges in the field of cell signal transducing by publishing the crystal structure of rhodopsin and the blocking protein complex in the journal Nature. This breakthrough was selected by academicians from both houses to select the top ten science and technology progress news in China in 2015, while Xu Huaqiang was awarded the Hans Neurath Award by the International Protein Society in 2016. In 2017, it again cracked the phosphate code for GPCR recruitment to deter proteins, and the results were published in the journal Cell in a cover article.
experts said that these results study the interaction between GPCR and downstream multiple effect proteins in depth and systematically, and expound the molecular mechanism of GPCR signal path conduction, which is of great theoretical significance and practical application value.
is understood to have been carried out by the Shanghai Institute of Drugs, the Winnello Institute, the Frederick National Cancer Research Laboratory, the University of Chicago, the University of Toronto in Canada and the National Cancer Institute. (Source: Huang Xin, China Science Daily)