Scientists have built a model of intestinal inflammation in zebrafish

The team of The Guangzhou Institute of Biomedicine and Health of the Chinese Academy of Sciences, Yu Tingqing and Shu Xiaodong, used zebrafish mutants to simulate part of the esoptery of inflammatory bowel disease, combined with in-body cell 3D culture results to explain its pathogenesis, and to build a intestinal inflammation model in zebrafish. The results were published online in
journal.
researchers used gene editing techniques to obtain two pyk3c3 mutants. Through histology, molecular biology, and electroscopy, the researchers identified the basic esophysics of intestinal inflammation in mutants. Studies have shown that the activation of the intestinal inflammatory response is not caused by microorganisms and the outside environment, but by autogenic damage to the intestinal cortical cells, which recruits a large number of neutral granulocytes. This is the biggest difference from the known zebrafish intestinal inflammation model.
to determine whether the root cause of the mutant's death was a defect in the immune system, the researchers decided to study the "clean intestine" in a petri dish. The Caco2 cell line is a human-sourced colon cancer cell. When a layer of substring glue is laid on the bottom of the Petri dish, a intestinal-like "doughnut" structure can be formed after 5 days. Inhibiting the expression of PYK3C3 under this system can also lead to the destruction of cell connections. In-body models further prove that the destruction of the cortical structure is the autonomy of the cortical cells.
addition, the team used zebrafish to discover the role of multiple vesicle transport proteins in early development, and PIK3C3 regulates the process of vesicle transport in the body through its catalytic products. (Source: Zhu Hanbin Huang Boquan, China Science Daily.)