[Science Sub-Journal] New breakthroughs in cell recombination programming | Cell therapy can help stroke patients repair their brains and is expected to restore 90% of their motor functions

This article is converted Medicine original, reproduced please indicate the source Author: chen Introduction: The existing method of treating stroke focused on re-open blocked blood vessels, but there is no way to solve the long-lasting damage to the brain caused by the stroke.

Recently, scientists at Ohio State University discovered that the use of tissue nanotransfection technology can "retrain" skin cells and repair damaged brain tissue.

Stroke is actually a common name.
It is not a disease, but an acute cerebrovascular disease.

It is mainly divided into two categories: hemorrhagic stroke (broken cerebral blood vessel) and ischemic stroke (blocked cerebral blood vessel).

Stroke is very common among the elderly, and its mortality rate increases with age.

One million people in China die from stroke each year, and the number of new or recurring stroke patients in the United States reaches nearly 800,000 each year.

Once a stroke occurs, it will cause defects in blood vessels and neuronal tissue.
Survivors often have irreversible brain damage, including paralysis, speech disorders, and partial loss of motor function.

Existing treatment methods for stroke focus on reopening blocked blood vessels, but there is no treatment method that can solve the long-lasting damage to brain tissue caused by stroke.

At the same time, recent studies have shown that therapies that only aim to promote endogenous tissue repair through pharmacological/nutritional factors are generally not efficient.

Therefore, there is still a need for effective therapies to reduce tissue damage and help repair after stroke.

Recently, scientists at Ohio State University (OSU) have made new breakthroughs in the field of cell reprogramming.

They used "tissue nanotransfection technology (TNT)" to effectively reprogram skin cells to turn them into vascular cells, and then injected them into the brain of stroke mice to promote the formation of new blood vessels, restore normal blood supply, and repair Damaged brain tissue.

The research was published in the journal "Science Advances", entitled "Nanotransfection-based vasculogenic cell reprogramming drives functional recovery in a mouse model of ischemic stroke".

Studies have shown that: the treated stroke mice recovered 90% of their motor functions and the damaged areas of the brain were repaired.
This means that tissue nanotransfection technology can "retrain" skin cells and then repair other damaged tissues.

TNT technology is based on nanochips to deliver biological cargo directly to the skin, thereby transforming adult cells into any cell type of interest for treatment in the patient's own body.

In mouse experiments, the researchers introduced a set of mixed transcription factor genes Etv2, Roxc2, and Fli1 (collectively referred to as EFF) into skin cells, and injected the reprogrammed cells into the damaged brains of stroke mice, and found These modified skin cells trigger the formation of new blood vessels in the brain, which can provide normal blood supply to tissues and help repair damaged brain tissue.

The research team found that the stroke mice that received this cell therapy recovered 90% of their motor functions.
Magnetic resonance imaging showed that the damaged areas of the brain were repaired within a few weeks.

Magnetic resonance imaging and behavioral tests showed that after intracranial injection of cells transfected with the EFF transcription factor combination nano-transfection, 70% of the stroke mice were dissolved and 90% of their motor functions recovered.

The results showed that the recovery ability of stroke mice was dose-dependent with the amount of nanotransfected fibroblasts with EFF gene combination perfused into the skull.

 Researchers have found that because the injected cells also release therapeutic signals in the form of vesicles, the mice can help restore damaged brain tissue and enable them to recover better.

The research results show that after intracranial injection of EFF nano-transfected fibroblasts, not only can they be transformed into vascular cells in the body, but also exosomal fluid can be released to mediate angiogenesis.

Researchers believe that this strategy may one day be used to help patients restore language, cognitive, and motor functions-even a few days after an ischemic stroke.

Dr.
Shahid Nimjee said: "We are looking for opportunities to restore the regenerative cells of the brain.

" Rewrite the genetic code of skin cells to make them blood vessel cells.

When they are deployed into the brain, they can grow new, healthy blood vessels to restore normal blood supply and help repair damaged brain tissue.

Cell reprogramming based on nanotransfection technology for cell therapy is a promising strategy for the treatment of ischemic stroke.

Reference materials: [1][2] https://advances .
sciencemag.
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