Science Journal: it is revealed that GDNF determines the fate of transplanted neural progenitor cells in vivo, which is expected to treat spinal cord injury

January 19, 2020 / Biovalley BIOON / - -- neural progenitor cells (NPC) is a potential treatment for repairing and regenerating neurons after spinal cord injury However, the harmful microenvironment in the injured spinal cord contributes to the limited recovery observed after NPC transplantation in rodents In a new study, researchers from institutions such as the University of Toronto in Canada found that Notch activation induced by spinal cord injury biased the fate of NPC transplanted into rodents towards astrocytes in the spinal microenvironment The relevant research results were recently published in the Journal of Science Translational Medicine, and the title of the paper is "GDNF reserves the fast of natural agent grants by attaching note signals in the involved spiral core in Rhodens" Picture from Science Translational Medicine, 2020, DOI: 10.1126/scitranslmed.aau3538 In the process of screening potential clinical factors regulating Notch signal, they identified glial cell-derived neurotrophic factor (GDNF) GDNF attenuates Notch signal by mediating the expression of DLK1 (delta like 1 homolog), which has nothing to do with the effect of GDNF on cell survival When transplanted into a rodent model of cervical spinal cord injury, human induced pluripotent stem cell-derived NPC (H IPS c-npc) expressing GDNF more differentiated into neurons than the control cells In addition, GDNF expression promoted the protection of endogenous tissues and enhanced the electrical integration of transplanted cells, thus jointly improving the recovery of neural behavior The effect of DLK1 knockout induced by CRISPR on functional recovery was weakened in H IPS c-npc expressing GDNF, which confirmed that the effect was partly mediated by DLK1 expression It can be seen that these results represent a mechanism driven optimization of hiPSC NPC treatment, thus redirecting transplanted hiPSC NPC to neuron fate and enhancing their integration In addition, they suggest that regulating the microenvironment of the injured spinal cord may improve the functional recovery after NPC transplantation (BIOON Com) reference: Mohamad khazaei et al GDNF reserves the fast of natural prognostor grants by attaching Notch signals in the adjusted spiral core in plants Science Translational Medicine, 2020, DOI: 10.1126/scitranslmed.aau3538