Sci Adv: The latest research results of the Chinese Academy of Sciences! R-ring structure may be able to coordinate with transcription factor Sox2 to regulate the pluripotency of cell reprogramming

June 14, 2020 /PRNewswire/ -- The special structure in R-loops cells, consisting of RNA-DNA hybrids and replaceable single-stranded DNA, is usually found near transcription genes, however, the R ring is also a dynamic and extensive physical structure that plays an ambiguous regulatory and epigenetic role in the genomePHOTO Source: CC0 Public Domain A recent study published in the international journal Science Advances, scientists from the Guangzhou Institute of Biomedicine and Health Research of the Chinese Academy of Sciences and other institutions through the study revealed the R ring in reprogrammed somatic cells into induced pluripotent stem cells (iPSCs) key roles and activities, the researchers found that the R ring structure can affect the reprogramming of somatic cells reprogramming iPSCs, interfere with the activity of the R ring structure or lead to the process of the transformation of the r-ring cells into ipsThe R ring may also play the role of epigenetic markers by altering the binding of transcription factors, the modification of chromatin, and methylation of DNA, in which the researchers mapped the Structure of the R ring during the reprogramming of oSKM-mediated somatic cells, while also timely ingesting the dynamic association between R-ring formation and decomposition and the process of reprogramming somatic cells, oSKM, four transcription factors in the reprogramming mix: Oct4, Sox2, And Klf4 and Klf4Researcher YAO Hongjie said the study data showed that R-rings can cause dramatic changes in both the early and late stages of reprogramming, but brief and subtle changes in the intermediate stages of reprogramming, similar to patterns of color openness, methylation of DNA, and gene expression during reprogrammingThe researchers found that some R rings form before gene expression, suggesting that the R ring prepares for gene transcription, which is closely related to the formation of the R ring structure complexFinally, the researchers say that Sox2 not only induces transcription factors that occur during the transcription process, it is also a necessary regulator to maintain the balance of the R ring structure, but also promotes the reprogramming process of cells together with the R ring structure(BioValleyBioon.com) Original origins: Yaoyi Li, Yawei Song, Wei Xu, et alR-loops with SOX2 in the revering reprogramming to pluripotency, Science Advances (2020)DOI: 10.1126/sciadv.aba0777