Restriction Enzyme Digestion, Gel Electrophoresis, and Vacuum Blotting of DNA to Nylon Membranes
-
Last Update: 2020-12-20
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
In 1975, Edwin Southern published a paper that revolutionized the molecular analysis of the genomes of organisms (
1
). This procedure enabled the detection of which fragment of
DNA
(out of the millions that compose the genome of a higher organism) contained sequences related to that of a radioactively labeled probe. It was thus possible to detect and genetically map restriction fragment length polymorphisms (RFLPs, reviewed in ref.
2
), to know how many copies of a gene were present in an organism, to map the restriction sites around a sequence of interest, and to check if a potentially transgenic organism had really integrated the input gene into its chromosome. Suitable selection of probes, so that they detected sequences that were highly polymorphic (
3
), enabled the identification of individuals from a population, and has led to the use of the method in forensic analysis. The identification of genes, or linked loci, affecting human disease, has also led to applications in clinical research.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
