Researchers develop new method for high-throughput screening of ketones

ketones are the basic compounds of organic chemistry and biocatalysis, and are important blocks for high-value chemicals such as pharmaceuticals and foodKetones have greater inertness than aldehydes and are often not suitable for ketones when they are displayedAt present, the reported ketone detection methods are characterized by low sensitivity, narrow base spectrum and limited detection environment, so it is very important to develop a high-throughput screening method for ketones with more broad spectrum and generality, the research team on enzyme molecular engineering and industrial biocatalytic led by Sun Zhoutong, a researcher at the TianjinIndustrial BiotechnologyResearch Institute of the Chinese Academy of Sciences, established a new high-throughput detection method for ketones through color rendering agent screening on this technical issueKetones of multiple structures can be quickly determined by using fluorescent dyes to metformin-2-aminocarinate (PMA)The detection method has the advantages of high sensitivity (sm level), strong fit (R 0.9), broad base spectrum (can detect more than 20 ketones in four categories), low background noise, color stability, etc., and can be used in whole cell or in vitro enzyme catalytic systemThe high-throughput display method was used for new enzyme mining and directional evolutionary screening based on ketone reduction and oxidation reaction, and new alcohol dehydrogenase and catalytic energy-boosting mutants were obtainedThe PMA-based fluorescence screening method developed in this study can be applied to the microporous plate screening of ketones, which provides a general high-throughput screening method for the discovery and modification of new biocatalysts based on ketones, and improves the screening efficiency(
Bio valleyBioon.com)