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Broccoli not only has a comprehensive nutritional composition, but also has a higher content of active ingredients such as glucosinolates and its derivative sulforaphane with health care effects than other cruciferous vegetables, and is known as
the "vegetable crown".
However, after harvest, broccoli has strong physiological metabolism, which is very easy to yellow, lose water and wilt at room temperature, and rapidly lose nutrients, resulting in a decrease
in its commodity value.
Although traditional preservatives can prolong the storage period of broccoli, there are secondary residues
.
Modified atmosphere preservation technology is green and safe, and is one of
the modern fruit and vegetable storage and preservation methods widely used at home and abroad.
Spontaneous modified atmosphere preservation is to seal fruits and vegetables in a container made of plastic film with specific breathable properties, using the respiration of fruits and vegetables themselves and the breathability of plastic film, under certain temperature conditions, self-adjust the O2 and CO2 content in the sealing environment to make it meet the storage requirements
.
Silicon window atmosphere control is a kind of spontaneous atmosphere control, which is embedded with a certain area of silicone rubber film on the packaging container, and uses the permeability of the silicone rubber film to select different gases to adjust the proportion
of gas in the packaging environment 。 Wei Lijuan and Feng Yuqin* of the Institute of Storage and Processing of Agricultural Products, Gansu Academy of Agricultural Sciences, and the Fruit and Vegetable Storage and Processing Technology Innovation Center of Gansu Province used cold-resistant excellent broccoli as the material, and proposed to process broccoli by setting different storage temperatures and silicon window area packaging, to explore the effects of storage temperature and silicon window area on the effect of broccoli silicon window controlled atmosphere preservation, and to determine the optimal parameter combination of spontaneous modified atmosphere of broccoli silicon window, in order to effectively inhibit the postharvest yellowing of broccoli and delay the quality deterioration of broccoli during storage.
It provides a theoretical basis
for the spontaneous modified atmosphere preservation technology of broccoli silicon windows.
1.
The effect of packaging bags with different storage temperatures combined with theoretical silicon window area value and ordinary PE packaging bags on the preservation effect of broccoli
Changes in respiration rate and relative conductivity
As shown in Figure 1A, broccoli belongs to respiratory transition vegetables, its respiration rate during storage generally shows a trend of first increasing and then decreasing, there are respiratory peaks (in terms of CO2, the same below), respiratory peaks occur in each group at 5 days, the respiratory peaks of the silicon window bag packaging treatment group are lower than those in the silicone-free window control treatment group, and the respiratory peak of the 2 °C silicon window bag packaging treatment group is the lowest, which is 26.
0%, 68.
4%, 36.
9% lower than the 4 °C silicon window, 4 °C silicon-free window, 2 °C silicon-free window, 0 °C silicon-free window, and 0 °C silicon-free window, respectively.
5.
1%、53.
8%
。 The respiration rate of each treatment group decreased to varying degrees after the peak of respiration, among which the respiration rate of the 2 °C silicon window bag packaging treatment group was the lowest at 10 and 20 days and at the end of storage, and significantly lower than that of other groups (P<0.
05) at 10 and 20 days, and the inhibition effect of 0 °C silicon window bag packaging treatment on respiration rate was inferior to that of 2 °C silicon window bag packaging treatment.
The respiration rate of the 0 °C silicone-free window group during storage was also greater than that of the 2 °C silicone-free window group as a whole, which may be caused by<b11> frostbite of broccoli due to systematic error dropping below the freezing temperature of broccoli.
The 4 °C silicone-free window packaging treatment group had the highest respiration rate throughout the
storage period.
It can be seen from Figure 1B that the relative conductivity of each treatment group gradually increased with the extension of storage time, among which the 4 °C silicone-window-free control group increased rapidly, and after 10 days, it was significantly higher than that of other treatment groups (P<0.
05), and the relative conductivity of the silicon window bag packaging treatment group was lower than that of the silicon-free window control treatment group<b10> in the late storage period 。 The relative conductivity of the 2 °C silicon window bag packaging treatment group was lowest after 5 days until the end of the storage period, and the 4 °C silicon window, 4 °C silicon-free window, 2 °C silicon-free window, 0 °C silicon window, and 0 °C silicon-free window were 11.
2%, 30.
1%, 12.
1%, 1.
2%, and 15.
9%,
respectively.
The relative conductivity of the 0 °C silicon window bag packaging treatment group increased faster than that of the 2 °C silicon window bag packaging treatment, and the 2 °C and 0 °C silicon-free window control groups had a similar change trend, because the low ambient temperature of the 0 °C treatment group caused damage to the broccoli cell membrane.
Changes in the volume fraction of oxygen and carbon dioxide in the bag
Silicon window can effectively adjust the gas composition in the packaging bag, from Figure 2, it can be seen that the change trend of O2 and CO2 volume fraction in each silicon window packaging bag is more stable than that in each silicon-free window packaging bag, silicone-free window packaging bag due to poor air permeability, the O2 volume fraction in the bag is sharply reduced, and the CO2 volume fraction accumulates too much, which is not conducive to broccoli storage
.
The O2 volume fraction of the 2 °C silicon window bag packaging treatment group within 5~25 d remained between 2.
3%~4.
1%, the volume fraction of CO2 remained between 8.
7%~11.
0%, and the gas ratio in the bag reached dynamic equilibrium, which was closest to the best storage gas environment
of broccoli.
Changes in chlorophyll, VC, soluble protein, and glucosinolates
During the whole storage period, the chlorophyll content of each silicon window bag packaging treatment group was higher than that of each silicone-free window treatment control group, among which the 2 °C silicon window bag packaging treatment group was the highest, and at the end of storage, it was higher than that of 4 °C silicon window, 4 °C silicone-free window, 2 °C silicone-free window, 0 °C silicon window, 0 °C silicon-free window, and 0 °C silicone-free window, respectively, 35.
4%, 174.
6%, 80.
7%, 16.
9%, and 68.
1%.
Among the three control groups at different temperatures, the chlorophyll content decreased the fastest in the 4 °C control group, and the 2 °C control group maintained the best
.
It can be seen from Figure 3B that the VC content of each group generally showed a trend of rising-decreasing, which may be due to the small increase in VC content of broccoli due to post-ripening in the early stage of storage, and VC began to decompose with the extension of storage time, and the relative content of VC increased in the later broccoli losing more water
.
The VC content of broccoli was the highest in the 2 °C silicon window bag packaging treatment group between 10~20 days, and the 0 °C silicon window bag packaging treatment group at the end of the storage period was higher than that in the 2 °C silicon window bag packaging treatment group, but there was no significant difference between the two (P>0.
05).
The VC content of broccoli in the 4 °C silicone-window-free control group was at the lowest level during the entire storage period, and was significantly lower than that in the 2 °C silicon-window bag packaging treatment group (P<0.
05), and the 2 °C silicone-free control group was higher than that in the 0 °C silicone-free control group<b14>.
As shown in Figure 3C, the soluble protein content of each group showed a gradual upward trend during the whole storage period, which may be the result of broccoli being subjected to low temperature storage and water loss stress, and the soluble protein content of the silicon window bag packaging treatment group during storage was higher than that of each silicone-free window treatment group, indicating that the silicon window bag packaging could maintain a lower metabolic strength
of broccoli 。 The soluble protein content in the 0 °C treatment group increased more than that in the 2 °C treatment group in the early stage of storage, and gradually lower than that in the 2 °C treatment group at the end of storage, indicating that the low ambient temperature of the 0 °C treatment group made broccoli synthesize related proteins to resist low temperature stress, but gradually damaged
with the extension of low temperature storage time.
It can be seen from Figure 3D that the glucosinolates content of broccoli in each treatment group first increased and then slowly decreased during storage, which may be caused
by the synthesis of indole glucosinolates and the degradation of aliphatic glucosinolates.
During the whole storage period, the glucosinolates content of silicon window bag packaging treatment group was higher than that of each silicone-free window treatment group, the lowest in the 4 °C silicone-free window group, the highest in the 2 °C silicon window bag packaging group, and significantly higher than that in other groups at the end of storage (P<0.
05).
<b18>
Changes in SOD and CAT vitality
As shown in Figure 4A, the SOD activity of each treatment group reached the first peak at 5 days, of which the 0 °C silicon window bag packaging treatment group was the highest, followed by the 2 °C silicon window bag packaging treatment group, the SOD activity of each group decreased after 5 days, and on the 20th day, the SOD activity of each group peaked again, and the SOD activity change showed a "bimodal curve", at this time, the 2 °C silicon window bag packaging treatment group was the highest, the 0 °C silicon window bag packaging treatment group decreased rapidly after this, and the storage end was significantly lower than 2 The SOD activity of the SiC Window Bag Packaging Treatment Group (P<0.
05), the 2 °C and 0 °C silicone-free control groups had a similar change trend, indicating that the SOD in the 0 °C treatment group first responded to low temperature stress, but the SOD activity was damaged<b10> to a certain extent with the extension of the stress time.
The SOD activity of the silicon window bag packaging treatment group was higher than that of the silicone-free window control group during the whole storage period, and the SOD activity of the silicone-free window control group at 4 °C was the lowest, indicating that the activation of SOD by silicon window bag packaging combined with low-temperature storage was more obvious
.
As shown in Fig.
4B, the CAT viability of each group showed a trend of first increasing and then decreasing, and the peak occurred in each group on the 15th day of storage, and the change trend of CAT activity with storage time in the 2 °C and 0 °C treatment groups was close to SOD viability, compared with the control group, the silicon window bag packaging treatment showed superiority
2.
The rationality of the theoretical silicon window area value is verified by the experimental results
Changes in respiration rate and relative conductivity
Respiratory peaks occurred on day 5 in all treatment groups (Figure 5A), and the peak value of the silicon window area 6 cm2 treatment group was the lowest, but with the extension of storage time, the respiration rate of the silicon window area 8cm2 silicon window bag packaging treatment group decreased significantly, and the respiration rate was significantly lower than that of other treatment groups after 15 days (P<0.
05).
<b11>
The relative conductivity of each treatment group gradually increased with the increase of storage time (Fig.
5B), among which the relative conductivity of silicon window area 8 cm2 silicon window bag packaging treatment group was the lowest throughout the storage period, and at the end of the storage period was significantly lower than that of other treatment groups (P<0.
05), indicating that the aging rate of broccoli in the silicon window area 8<b14>cm2 silicon window bag packaging treatment group was relatively slow
during storage.
Changes in the volume fraction of oxygen and carbon dioxide in the bag
As shown in Fig.
6A, the O2 volume fraction in the bag of the silicon window area of 8 cm2 silicon window bag packaging treatment group after 5 days of storage was the closest to the optimal O2 volume fraction range for broccoli storage, and the change was stable.
The O2 volume fraction in the bag was stable but not close to the optimal volume fraction range in the silicon window area 6cm2 silicon window bag packaging treatment group, and the O2 volume fraction in the silicon window area 10cm2 treatment group was close to the optimal volume fraction range but the change trend was not stable
.
The control group consumed O2 for respiration and the O2 volume fraction in the package was too low due to poor air permeability of the packaging bag, which was not conducive to broccoli storage
.
As shown in Figure 6B, the CO2 volume fraction in the silicon window bag packaging treatment group after 5 days of storage was close to the optimal CO2 volume fraction range for broccoli storage, and the change trend of 8 cmtwo groups was the most stable, indicating that the CO2 volume fraction in the bag reached dynamic equilibrium
.
The control group produced CO2 from respiration of broccoli, and the CO2 volume fraction in the packaging bag was too high due to poor air permeability, which was not conducive to broccoli storage
.
Changes in chlorophyll, VC, soluble protein, and glucosinolates
7A that the chlorophyll content of broccoli in each treatment group showed an overall downward trend during storage, among which the silicon window bag packaging treatment group with a silicon window area of 8 cm2 was the highest, which was significantly higher than that of other groups (P<0.
05) during 0~20 d, and higher than that of 10 cm2 group 2.
85% at the end of storage period, significantly higher than that of 6<b10>cm2 group and control group (P<0.
05).
<b11>
It can be seen from Figure 7B that the VC content of the silicon window bag packaging treatment group with a silicon window area of 8 cm2 after storage for 10 days was the highest, which was 3.
6% higher than that of the 10 cm2 treatment group at the end of storage, and significantly higher than that of the 6cm2 group and the control group (P<0.
05).
<b15>
It can be seen from Figure 7C that the soluble protein content of the silicon window bag packaging treatment group with a silicon window area of 8cm2 during storage has been higher than that of other treatment groups, and the difference was significant at the end of the storage period (P<0.
05).
<b19> The soluble protein content of the control group was significantly lower than that in the silicon window bag packaging treatment group with an area of 8cm2 (P<0.
05)<b111> during the whole storage period.
It can be seen from Fig.
7D that the glucosinosides content of broccoli in each treatment group first increased and then decreased during storage, and the glucosinoside content of the silicon window bag packaging treatment group with a silicon window area of 8 cm2 was the highest throughout the storage period, and was significantly higher than that of other treatment groups (P<0.
05) on day 10, and 30.
1% higher than that of 6<b112>cm2 group, 10 cm2 group and control group at the end of storage period, respectively.
、11.
3%、94.
5%
。
Changes in SOD and CAT vitality
As shown in Fig.8A, the SOD viability change showed a "bimodal" curve, and the SOD viability of each treatment group reached the first peak when stored for 5 days, fell back after 5 days, and the second peak occurred when stored for 20 days, at this time, the silicon window bag packaging treatment group with a silicon window area of 8 cm 2 was the highest, and the difference from other treatment groups was significant (P<0.
05); the SOD activity of the silicon window bag packaging treatment group with a silicon window area of 8 cm<b10>2 was the highest during 10~25 days
。 As shown in Fig.
8B, the CAT activity of each group peaked on the 15th day of storage, and showed a trend of first increasing and then decreasing, and the CAT activity of the silicon window bag packaging treatment group with a silicon window area of 8 cm2 was the highest during the whole storage period, which was significantly different from other groups at 10 and 15 days (P<<b14>0.
05), and was higher than that of 6 and 10cm2 silicon window bag packaging treatment group and control group by 16.
5%, 6.
8% and 35.
5%, respectively.
。
Conclusion
The screening results of the key parameters of spontaneous atmosphere control of broccoli silicon window show that the storage effect of spontaneous atmosphere control of silicon window is better than that of ordinary silicone-free window.Considering the bad preservation effect that may be caused by the temperature error of the storage environment, the broccoli storage temperature is 2 °C, and the ratio of mass to silicon window area is 5 kg/8cm2 to have the best preservation effect
.
The results of the verification experiment show that the preservation effect of the packaging bag with a silicon window area of 8 cm2 is better than that of 6 cm2 and 10cm2 at the most suitable storage temperature of 2 °C, and the setting of the theoretical silicon window area value in the parameter screening experiment is reasonable
。 The determination of the final dynamic equilibrium point of O2/CO2 in the spontaneous modified atmosphere system of broccoli silicon window is the key to the system design, if the system parameters are unreasonable, too low O2 volume fraction and too high CO2 volume fraction inside the package, which will cause fruits and vegetables to be in an anaerobic state or cause carbon dioxide damage, which cannot achieve the purpose of extending the storage period of
fruits and vegetables.
In this study, the ratio of broccoli mass to silicon window area was 5 kg/8cm2, and stored at 2 °C had the best preservation effect, which had certain practical significance for delaying the yellowing of broccoli aging, improving broccoli storage quality, and guiding the spontaneous controlled atmosphere storage of broccoli silicon windows, which provided a theoretical basis
for the spontaneous atmosphere control of broccoli silicon windows.
About the corresponding author
Feng Yuqin, researcher, director
of the Research Office of Processing Raw Materials and Quality Control, Institute of Storage and Processing of Agricultural Products, Gansu Academy of Agricultural Sciences.
Education:
D.
; (2) 2000-09 to 2003-06, Gansu Agricultural University, Forage Breeding, Master; (3) 1987-09 to 1991-06, Gansu Agricultural University, Horticulture, B.
S.
Scientific research and academic work experience: (1) 2017-01 to present, Researcher, Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences; (2) 2007-01 to 2016-12, Associate Researcher, Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences; (3) 1991-07 to 2006-12, Institute of Food Crops, Gansu Academy of Agricultural Sciences, None
.
About the first author
Wei Lijuan, Assistant Researcher, Institute of Storage and Processing of Agricultural Products, Gansu Academy of
Agricultural Sciences.
.
Scientific research and academic work experience (except postdoctoral work experience):(1) 2018-12 to present, Assistant Researcher, Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences; (2) 2015-01 to 2018-11, Research Intern
, Institute of Agricultural Products Storage and Processing, Gansu Academy of Agricultural Sciences.
This article "Screening and verification of spontaneous modified atmosphere silicon window area of broccoli silicon window based on temperature conditions" is from Food Science, Vol.
43, No.
15, pp.
236-244, 2022, authors: Wei Lijuan, Feng Yuqin, Li Cuihong, Li Changliang, Yu Jiawen
.
DOI:10.
7506/spkx1002-6630-20210729-339
。 Click to view information about
the article.