Research progress in the pharmacological effect of Coptis chinensis on PU

Business Club reported on February 29 that Coptis has multiple medical effects In recent years, it has been found that Coptis chinensis has a unique anti peptic ulcer (PU) effect At present, the main research work focuses on Coptis chinensis formula and its main components BER and berberine The effect of Coptis chinensis and its formula on Pu experimental model was studied According to Gao binyingshu (1988) and takaseh (1990), the dried water extract powder of Coptis chinensis and Huanglian Jiedu Decoction (Coptis, Scutellaria, phellodendron, Gardenia jasminoides Ellis) administered by gavage with a weight of 25-100 mg / kg can inhibit the decrease of gastric mucosal potential difference caused by ethanol in a dose-dependent manner Yongdingguo et al, School of medicine, Zhejiang University, found that Zuojin Pill (huanglianwuzhuyu = 6 × 1) 0.3 g / kg body weight, 0.75 g / kg body weight, 1.05 g / kg body weight gavage had protective effect on ethanol induced gastric mucosal injury, the inhibition rate of 1.05 g / kg body weight ulcer was 26.2%; the inhibition rate of 1 g / kg cimetidine was 55.3% Chen Weiwen, et al Of Guangzhou University of traditional Chinese medicine, found that Zuojin Pill (huanglianchen, Wuzhuyu, Muxiang = 6 × 1 × 1), 1g / kg body weight, 2G / kg body weight and 4G / kg body weight can significantly inhibit the formation of water immersion stress gastric ulcer, and the inhibition rate of 4G / kg dose group was 66.86% Chen Yanfen et al Reported that Zuojin Pill 0.3 g / kg body weight, 0.75 g / kg body weight, 1.05 g / kg body weight can inhibit indomethacin induced gastric ulcer in a dose-dependent manner The inhibition rate of 1.05 g / kg body weight is 41.7%, and that of cimetidine 1 g / kg body weight is 59.7% These results show that Coptis can significantly enhance the gastric mucosal barrier function, and has a significant preventive and therapeutic effect on acute and chronic gastric ulcer in rats, but the effect is relatively mild The protective effect may be the result of synergism of alkaloids in Coptis chinensis Effects on gastric acid, pepsin and gastric mucosa HCO3 barrier Chen Weiwen et al Found that the compound preparation of Coptis chinensis Zuojin Pill (2.8g / kg body weight) was given to rats with pylorus ligation through duodenum, which showed that the gastric juice volume, total acid output and pepsin activity were significantly reduced except for the gastric acidity, in which the gastric acid secretion was reduced by more than 100% Jiawei Zuojin Pill 4G / kg body weight can also significantly inhibit the secretion of basic acid and gastrin induced high gastric acid, with the inhibition rates of 83.6% and 53.0%, respectively The above results suggest that Coptis chinensis and Coptis chinensis as the main medicine have a very significant inhibitory effect on gastric acid secretion By using the corne method, Chen Yanfen et al Also found that Zuojin Pill (2.8g / kg body weight) can increase the gastric wall binding mucus (AM) of rats by gavage, with an increase rate of 10.08%, while the increase of AM synthesis may enhance the barrier function of gastric mucus HCO3 - The effect on gastric mucosal blood flow According to the research of Cao Jiwang and Wang Wenya in the people's Hospital of Wuhan University, Coptis can dilate blood vessels and increase the blood flow of gastric mucosa In recent years, many domestic experts have combined the anti ulcer drugs with berberine, the main component of Coptis chinensis, to treat HP infected Pu, and achieved satisfactory results Mengqingling, Nanjing Pukou hospital, Jiangsu Province, combined omeprazole and BER in the treatment of PU with HP positive patients, 84 cases in the treatment group and 84 cases in the control group Results the ulcer healing rate in the treatment group and the control group was 96.3% and 97.10%, 89.4% and 90.0% respectively, suggesting that Coptis chinensis can not only remove HP infection, but also has fewer side effects, and has better effect on improving pathology and symptoms The mechanism of anti Pu action of Coptis chinensis is still unclear, which may be related to the following mechanisms: inhibition of HP HP was positive in 70% of patients with chronic gastritis and gastric ulcer Wang Xulin, General Hospital of Tianjin Medical University, studied 200 kinds of traditional Chinese medicine and found that Coptis, Scutellaria, rhubarb and other antibacterial effects were obvious, among which Coptis had the largest antibacterial ring of 25 mm The above results suggest that Coptis chinensis has a very significant effect on the inhibition of HP, which is related to its potential anti Pu effect Regulation of intracellular free calcium level It has been confirmed that BER can inhibit the increase of Ca2 + concentration in nerve cells, myocardial cells, gastrointestinal smooth muscle cells, etc Coptis can inhibit the gastric mucosa damage caused by the increase of intracellular free calcium under stress, which is related to its protective effect on gastric mucosa Effects on the autonomic nervous system The autonomic nervous system regulates gastrointestinal motility, blood supply, gland secretion and gastric mucosal barrier function The disorder is closely related to the pathogenesis of PU Animal experiments show that Coptis alkaloids can improve gastric mucosal blood flow and enhance gastric mucosal barrier at receptor level, but the inhibition of gastric acid secretion may not be related to receptor Satohk et al Supported this argument and found that Coptis alkaloids, another major component of Coptis chinensis, can significantly inhibit proton pump, with IC50 between 0.16 and 0.79 mmol / L The regulation of IL-8 on cytokines involved in inflammation is the most powerful chemokine and activator of polymorphonuclear leukocytes, which can reflect the degree of gastric mucosal damage to a certain extent The mechanism of BER's anti mucositis may be related to the following aspects: inhibiting the production and secretion of IL-1, TNF - α, IL-2, IFN - γ, IL-8 and other cytokines; inhibiting iNOS, so as to inhibit the secretion of these cytokines, inhibiting the high expression of ICAM-1 and CD18 on the surface of endothelial cells; inhibiting the adhesion between lymphocytes and endothelial cells, and blocking lymphocytes Recycling, reducing the chance of contact with antigen, also inhibiting the contact of endothelial cells as antigen presenting cells with T cells, affecting the activation of T cells; inhibiting neutrophil phospholipase A2 (PLA2), reducing the production of PGE2 in inflammatory tissues, helping to stop or slow down the inflammatory response Anti reactive oxygen species In vitro experiments showed that Coptis chinensis had the effects of anti lipid peroxidation, inhibiting the production and elimination of free radicals Due to the production of a large number of reactive oxygen species in the gastric mucosa under the conditions of chemical substances, ischemia or cell energy deficiency, which damage the gastric mucosa, leading to the formation of ulcers Pharmacokinetic study of gastric mucosa Expert research points out that Coptis alkaloids have a unique distribution in gastric mucosa It was found that the absorption dose of Coptis alkaloids (50-400 mg / kg) increased with the increase of the dosage, and there was a good positive correlation between them 15 minutes after the administration, the concentration of alkaloids in the gastric mucosa reached the peak According to the first-order kinetic calculation, the half-life of alkaloids in the gastric mucosa was 194 minutes The alkaloids absorbed rapidly in gastric mucosa and maintained high concentration in gastric tissue for a long time, which may be helpful for Coptis to play a local role in gastric mucosa In a word, Coptis chinensis and its total alkaloids are promising drugs for the treatment of PU and chronic gastritis Further research and development are needed.