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MicroRNA plays an important role in the development and functional regulation of the nervous system, and is an indispensable part of its normal operation.
miRNA depression
In this study, the authors used CRISPR/Cas9 technology to construct miR-218-1 and miR-218-2 gene knockout mice, revealing that miR-218 is widely expressed in the brain, especially the highest expression level in the hippocampus; and After knocking out the miR-218-2 gene, the expression level of miR-218 in the hippocampus decreased significantly.
In order to clarify the molecular mechanism of cognitive deficits in miR-218-2 knockout mice, the authors used whole-cell patch clamp technology, Golgi staining and cryo-electron microscopy to analyze the development, morphology, and morphology of miR-218-2 knockout neurons.
Furthermore, the authors combined RNAseq and miR-218 target gene prediction data and performed KEGG analysis to screen out possible miR-218 target genes.
In summary, the miR-218-2 gene in the mouse hippocampus regulates the morphology of neurons and the transport and release of synaptic vesicles through the target gene C3, thereby affecting the synaptic reversibility of neurons and the occurrence of LTP.
