Research and development of hivelisa detection reagent with polypeptide as antigen
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Last Update: 2015-07-09
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Source: Internet
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Author: User
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Recently, researchers from the laboratory of the First Affiliated Hospital of Guangxi Medical University published a paper to prepare a kit for the detection of HIV-I / II antibody by using four specific synthetic peptide antigens (HIV-I gp41, gp120, p24 and hiv-ii gp36) as the main raw materials for coating The results show that it is feasible to prepare a kit for detection of HIV antibody with chemically synthesized polypeptide antigen as the main coating material The main active components of the kit are stable and have good sensitivity and specificity This article was published in the 10th issue of Chinese new clinical medicine in 2014 Using chessboard titration to select the appropriate concentration of antigen coating and working concentration of enzyme labeled antigen, after optimizing the detection system, the performance of this kit was verified, and Shanghai Kehua, Xiamen Yingchuang and Zhuhai Lizhu reagents were used as the control The results showed that the suitable concentration of coated antigen was 5 μ g / ml, and the working concentration of enzyme labeled antigen was 1:160 After optimizing the detection system reagent, cut-off value is 0.207, sensitivity is 97.2%, specificity is 98.6% The stability and repeatability of the main active components of the reagent are good, which are consistent with the test results of the control reagent.
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