RescueMu Protocols for Maize Functional Genomics
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Last Update: 2021-01-19
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Source: Internet
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Author: User
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RescueMu
is a modified
Mu1
transposon transformed into maize to permit mutagenesis and subsequent recovery of mutant alleles by plasmid rescue.
RescueMu
elements insert late in the germline as well as in terminally dividing somatic (e.g., leaf) cells. Germinal insertions may result in a mutant phenotype, and
RescueMu
permits recovery of 5–25 kb of transposon-flanking genomic
DNA
without having to construct and screen genomic DNA libraries. Late somatic insertions of
RescueMu
do not result in a visible phenotype, but they are instead used to construct plasmid libraries of gene-enriched maize genomic DNA to facilitate the identification and sequencing of the euchromatic portion of the maize genome. This is because maize leaves contain abundant independent
RescueMu
somatic insertions, and 70–90% of these insertions occur preferentially into genes and not repetitive DNA. This chapter describes detailed protocols on how to obtain, generate, and use
RescueMu
for maize genomics, including resources developed by the Maize Gene Discovery Project (MGDP) consortium available online at ZmDB.
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