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Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group

 Last Update: 2021-03-09
 Source: Internet
 Author: User

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In this chapter, we describe a real-time
PCR
detection system for fast, reliable, specific, and sensitive detection and discrimination of
‘Candidatus Phytoplasma mali’
,
‘Ca
.
P. prunorum’
, and
‘Ca. P. pyri’
from the 16SrX (apple proliferation-AP) group. These phytoplasmas are causal agents of fruit tree diseases within the
Rosaceae
family, namely apple proliferation, European stone fruit yellows, and pear decline. The assays use (hydrolysis) TaqMan
�
minor groove binder probes. The panel of assays comprises the same set of primers and specific probes for species-specific amplification, and an additional set of primers and probe for 18S rRNA as an endogenous quality control of
DNA
extraction. The assays described can be used in routine phytoplasma surveys and in certification programmes.

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