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Rapid and High-Throughput N-Glycomic Analysis of Plant Glycoproteins

 Last Update: 2021-03-07
 Source: Internet
 Author: User

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mass spectrometry principle

plant cell culture

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Glycoprotein is a major element in higher organisms including mammalians and plants. It is widely accepted that variation in cellular
N
-glycome is related to modulation in dynamic cellular mechanisms such as cell-cell adhesion, cell activation, and malignant alterations in mammalian cells. However, the physiological importance of glycan modification of glycoproteins in plant cells is still a matter of dispute. Therefore, a comprehensive and high-throughput analysis of
N
-glycome in plant glycoproteins is needed. Here, an application of the glycoblotting-mass spectrometry technique to plant glycoprotein research is described.

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