Progress was made in the study of the biosynthetic and analgesic activity of flavonoid carbonosine.

On March 6, the Wang Yong Research Group of the Center for Excellence and Innovation in Molecular Plant Science of the Chinese Academy of Sciences published a research paper entitled Pathway-specific enzymes from bamboo and crop leaves biosynthesize anti-nociceptive C-glycosylated flavones online.
The study revealed the distribution law and synthesis process of carbon glycoside flavonoids in the plant, and finally realized the head-to-head synthesis of rare flavonoid carbide in the microbial chassis, and revealed the unique anti-inflammatory analgesic mechanism of flavonoidgrass glycoside.
bamboo is a single leafy undergraduate plant, with a long history of medicinal food, is considered to be able to "clear the heat to remove annoyance, raw urine."
in bamboo leaves is its main medicinal substance, but its biosynthetic and pharmacological mechanisms lack in-depth study.
study looked at the rare carbon glycoside flavonoids in bamboo leaves.
is rare in nature compared to the common oxycoside flavonoids, but its structure is more stable and has more pharmacological activity.
study of the genealogy of flavonoids in the undergraduate plant in this paper, it is found that the compound has its evolutionary distribution law in different undergraduate plants.
crops of PACMAD branches such as corn and grain mainly produce flavonoid monosaccharides, while the BOP branches (rice, wheat, etc.) mainly produce flavonoids bisaccharides.
By comparing genomic analysis, the researchers found 32 UCT708A family glycosyl transferases that may be involved in the biosynthetic synthesis of flavonoid carbide in the genome collinear regions of rice, bisorb grass, bamboo, wheat, corn, sorghum, and milligens.
further evolutionary analysis found that these glycosyl transferases can be divided into two branches, A and B.
in-body functional verification showed that these UGTs were involved in carbon glycoside glycosylation of the flavonoid carbon glycoside pathway intermediate 2-hydroxy grapefruitin.
where branch A is a strict UDP-glucose-based glycosyl transferase, while branch B is hybrid in the identification of glycosyl supply, can identify both UDP-glucose and UDP-Arabic sugar.
researchers dug deeper into other enzymes involved in the synthesis of rare carbon glycoside flavonoids in the plant.
unearthed two unknown flavonoid oxycodone enzymes from the bamboo genome and integrated them into E. coli chassis cells with the grapefruit prebiotic synthesis module and the carbon glycosylation module.
By using an orthosis of flavanone 2-bit hydroxylase and flavanone 3' hydroxyase from different species (rice, corn, sorghum, bamboo, athropobacteria) sources, the researchers were successful in achieving
The head-to-head synthesis of four rare flavonoids, such as grassin, iso-glycoside, peony serin and iso-peony glycoside, reached the level of 20 to 30 mg/L in the shaker bottle.
further evaluated the activity of these carbon-glycoside flavonoid monomers and found that isoorientinhad significant pain inhibitory activity.
the analgesic effect of the pain-escrow test I phase in mice was comparable to that of the positive control drug Rotonin, and the analgesic effect of the II phase was comparable to that of the positive control drug aspirin.
further target experiments have found that iso grass glycosides can inhibit the production of Folmarin-induced TNF-alpha, and increase the production of pain-resistant allergic cytokine IL-10 in a dose-dependent manner to play an anti-inflammatory analgesic effect, through the target TRPV1 and NR2B signaling path to achieve neuropulation analgesics.
the study expounded the synthesis path of rare flavonoid carbide in bamboo and other plants, and evaluated the anti-inflammatory analgesic effects of these carbon glycoside monomers, and revealed their possible pharmacological mechanisms.
laid the foundation for the in-depth development of the plant resources and the rare active ingredients contained therein.
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