Progress was made in the study of nighttime light-induced negative emotions.

It is the first time that the circadian circadian rhythm, or circadian rhythm, can be induced by the circadian circadian rhythm, but not by the Chinese Academy of Sciences Rhythm gated modulation, for the first time, explains the internal mechanism of the opposite emotional effects of light in day and night.related results were published online in nature neuroscience on June 1, under the title of a circular RH gated subcortical pathway for nighttime light induced depressive like behaviors in mice.light is the basis of life. Life on earth evolves under the alternating light conditions of day and night, and evolves a series of neurophysiological functions of light perception and light regulation, in addition to image vision Besides, it also includes a series of physiological functions called "non image forming vision", such as light regulation of biological clock, sleep, photophobia, pupil light reflex and melatonin expression.in the thousands of years of human evolution, there is really a rich night light, which is only a hundred years after the industrial revolution, and evolution is far from enough to make human adapt to the changes of artificial light environment.a series of public health evidences show that long-term exposure to abnormal night light can lead to depressive mood and cognitive impairment, but we know little about the underlying neural mechanism.recently, the research on the neural loop of light regulating emotion has become a hot spot. A study (Fernandez et. al., cell, 2018) showed that long-term fragmented light dark stimulation may induce negative emotions through the projection from the retina to the perihabenular nucleus (PHB).however, it is still unknown whether the disturbance of light or rhythm affects emotion, and the complete light controlled emotional neural circuit has not been described.more importantly, the light of the day often positively regulates human emotions (Huang et. al., neuron, 2019). The principle behind the effect of the opposite light of day and night on emotion has always been an unsolved mystery.to answer the above questions, Xue Tian's research group constructed a light at night (LAN) model to simulate the abnormal light patterns in the current society.for three weeks, blue light irradiation for 2 hours from 9:00 to 11:00 every night can make mice show depression like behavior without disturbing rhythm or sleep.non imaging visual function is considered to be mediated by a class of photoreceptor cells in the retina, which are different from the cone rod cells.by using rod cells, cone cells and iprgc deficient transgenic animals, the research group found that iprgc played a full and necessary role in the process of LAN induced depression.in order to further reveal the central structure that mediates the action of LAN, the research group found that iprgcs projected to PHB. After PHB was damaged, the emotion related behavior of mice was no longer affected by LAN.at the same time, using photogenetic activation of iprgc to project PHB, can also induce depression like performance in mice.these results fully verify that PHB is an important loop node mediating the role of LAN.further loop studies showed that the dorsal part of PHB (dphb) projected to the nucleus Accumben (NAC), while the ventral part (vphb) projected to the medial prefrontal cortex (mPFC).NAC is closely related to reward, while mPFC is closely related to emotion and advanced cognition. the study group inhibited the input of PHB to mPFC and NAC respectively through AAV virus tool. The results showed that only the inhibition of dphb NAC projection could affect the effect of LAN. for three weeks at the same time, the projection of dphb NAC was directly activated at a specific time of night, which did not depend on the night light to induce depression like performance. These evidences reveal the core role of dphb NAC projection in LAN induced negative emotions. finally, in order to further explore the mechanism of different regulation of light on emotion in different periods of time (day / night). using single cell patch clamp and in vivo optical fiber recording, the researchers found that dphb neurons projecting to NAC were more excitable and more likely to release action potentials at night than during the day, while vphb neurons projecting to mPFC had no similar phenomenon. these results indicate that dphb neurons projecting to NAC, as a valve controlled by circadian rhythm, only open at night, allowing nocturnal light signals to regulate NAC, which is closely related to reward, through dphb, thus inducing negative emotions. further study suggested that this neural circuit of iprgcs dphb NAC might avoid the animals from being exposed to light during the alternation of day and night under normal physiological conditions, thus avoiding natural enemies. if this loop also exists in human beings, it may be hijacked by artificial light environment at night, thus inducing negative emotions. this study is the first time to elaborate the loop basis and rhythm gating mechanism of nocturnal light induced negative emotions. These findings can, to a certain extent, suggest the mechanism of negative emotions such as depression caused by night light interference (the use of electronic devices such as city lighting or mobile phones and computers), which is important for people to correctly understand the potential hazards of nighttime excessive lighting and explore prevention and treatment measures significance. Dr. an Kai of Xue Tian's research group and Zhao Huan, Professor of Hefei University, are the co first authors, and Zhao Huan and Xue Tian are the co authors. the research was supported by NSFC, the "973" program of the Ministry of science and technology, and the class a pilot project of Chinese Academy of Sciences. China University of science and technology has made progress in the study of negative emotions induced by night light