Progress in the study of the classification and systematic evolution of ginseng plants

Ginseng is a small genus of the Five Gaco, with about 10-18 speciesAmong them, as a well-known medicinal plant of 37 (Pnotoginseng), has not been found in the wild population, so its wild proximity is the 37 species improved potential resource treasureHowever, due to the conservativeness of reproductive organs and the diversity of nutritional organ morphology, the classification and species identification of 37 wild close species has been difficult and controversialRecently, the Kunming Plant Research Institute of the Chinese Academy of Sciences featured the selection and industrialization of new species breeding and industrialization demonstration team species endangered mechanism and group genetics research group, distributed in southeastern Yunnan and surrounding areas of ginseng plants in the field resources survey and material collectionBased on the multigene sequence data, the collected ginseng plants were clustered and combined with morphological traits, and the classification, kinship and genetic diversity of sanseven and its wild close relatives were discussed, and the results showed that: (1) screen edge 37 (Pstipuleanatus) and stee7 easily differ from other close proximity species, while the traditional bamboo section of the category of the class, genetic variation, complex genetic components, morphological characteristics of transitional variation, difficult to distinguish and identify; Some samples of zingiberensis) and bamboo verses, in the root stem or small leaf form are not consistent with the characteristics described in Chinese plants, but the comprehensive analysis of DNA data has determined the classification group properties of these groups; The genetic diversity of its wild proximity species is relatively highThe above findings are published in the Genetic Resources and Crop Evolution issue under the panax species with the help of THE DNA dataIn ginseng genus, the feather leaf 37 complex group (PThe bipinnatifidus complex contains other ginseng plants except Ppseudoginseng, 37 and screen-side 37, which are distributed in the Himalayas and central and southern ChinaThe complex group overlapped in southwest China and its surrounding areas, and the main classification characteristics among the groups were the difference between root stems and leavesHowever, due to the often transitional variation of these characteristics, there are still great problems and difficulties in the actual identification work and taxonomic researchPrevious studies based on DNA molecular fragments or AFLP markers do not strongly support the classification status of species or variants by morphological characteristics of different classification systemsThe team simplified genome sequencing (ddRAD-seq) for 100 individuals at 18 collection points of the feather leaf 37 complex in southwest China, and the close proximity of the ML tree shown based on RAD data basically matched with the existing classification processing: that is, ginger-shaped three-seven, Vietnam's three-seven (PVietnamensis) each branched into a single line, and the two formed sister groups, and for the traditional bamboo slug sage groupWangianus branch, root-shaped beaded cluster of p Bipinnatifidus branch The results of this cluster analysis show that in the classification of these groups, the form of root stems may be more reliable than the leaves In addition, the results of the group analysis results of network, STRUCTURE, PCoA and D-statistic combined group are inferred that there is a certain degree of genetic differentiation among the different groups of the complex, the genetic differentiation between the groups with geographical isolation is significant, and there is no local genetic communication between the groups with obvious geographical isolation The above findings are published in Molecular Phylogenetics and Evolution, panax bipinnatifidus complex in Southwestern China by RAD-seq data Ph.D candidate Zhou Mingmei is the first author of the paper, and researcher Yu Yu and senior engineer Pan Yuezhi are the communication authors (BioValleyBioon.com)