Progress in the study of in vitro metabolism of Chinese medicine extracted by Chengdu Institute of Biology

Due to the complexity of metabolism of traditional Chinese medicine and the limitation of analytical methods, most of its in vitro metabolism studies focus on the metabolism of one or some active ingredients However, traditional Chinese medicine is a complex mixture of many components The metabolism of one component or the simple superposition of each component cannot reflect the real situation of traditional Chinese medicine metabolism Therefore, the study of the whole metabolism of Chinese herbal extracts may provide a new idea for this proposition In order to study the whole metabolism of traditional Chinese medicine in vitro, it is necessary to solve the problems of the efficiency of metabolism in vitro and the separation and analysis of complex chemical components The researchers of Chengdu Institute of biology, Chinese Academy of Sciences used magnetic nanoparticles as the stationary phase to fix the liver microsomes on its surface to prepare a bioreactor, which has the advantages of high metabolic efficiency, convenient pretreatment of sample analysis, and can be used repeatedly The bioreactor, combined with HPLC-MS and other structural analysis methods, has great advantages in the study of the overall metabolism of Chinese herbal extracts After incubation of Coptis chinensis extract with the bioreactor, magnetic separation was used to separate the bioreactor and the reaction solution; the reaction solution was directly detected by UPLC-MS / MS, and a series of reactants (the intrinsic components of Coptis chinensis) and metabolites were identified Two major metabolites, normethylene berberine and fentanyl, were found Middle demethylidene berberine is a common metabolite of berberine, palmatine, jatrorrhizine and tetrandrine Finally, the metabolic pathway of the main components in the whole metabolism of Coptis chinensis extract was proposed This study studies the metabolites, metabolic pathways and the relationship between enzyme induction and inhibition of traditional Chinese medicine from a holistic perspective, showing a broad application prospect in the study of the metabolism of traditional Chinese medicine mixture, the interaction of traditional Chinese medicine compatibility and the basis of active substances The research results were published in anal bional Chem, 2013, 405: 8807-8817