Progress in the construction of multi enzyme complex to improve the electricity production efficiency of cellulose

Cellulose is the most abundant renewable resource on the earth, which can be used to produce biofuels and bio based chemicals Compared with the traditional microbial fermentation, the in vitro multi enzyme system has been successfully applied to the catalytic conversion of cellulose to inositol However, due to the high activation energy of the reaction pathway, the low specific activity of key enzymes, and the poor pulling ability of the downstream reaction, the initial reaction speed and conversion efficiency of the whole reaction system are still limited Recently, the research team led by you Chun and Zhu Zhiguang, researcher of the center for in vitro synthetic biology of Tianjin Institute of industrial biotechnology, Chinese Academy of Sciences, has improved the power generation efficiency of the whole reaction system by building a multi enzyme complex in the in vitro multi enzyme system for cellulose electricity generation In the early design, cellulosic biomass was made by using in vitro multi enzyme system Based on the enzymatic phosphorylation pathway of stoichiometric transformation, the team constructed different types of multienzyme complexes (Fig a) with the speed limiting enzyme (oligosaccharide phosphorylase) in the pathway and the downstream cascade enzyme (glucose phosphorylase) The optimal multienzyme complex can accelerate the reaction rate of glucose-6-phosphate phosphorylation of cellulose, which can be increased by 2.4 times, and the multienzyme can be Compared with the free enzyme system, the current density and power density of the complex were increased by 3.35 times and 2.14 times respectively (Fig B, c) This study is the first report of bioelectricity production by cellulose, and provides a new way to improve the initial reaction speed and conversion efficiency of multi enzyme system in vitro The research is supported by key deployment projects of Chinese Academy of Sciences and National Natural Science Foundation of China Relevant research results have been published in biotechnology for biofuels Meng Dongdong, assistant researcher of Tianjin Institute of technology, is the first author of the paper, and you Chun and Zhu Zhiguang are co correspondents of the paper (BIOON Com)