Progress in quantitative proteomics by Chinese scientists

Beijing, Dalian, September 11 (guanjianing) Pan Yanbo) Zou Hanfa research team of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, has made progress in new technologies and methods of quantitative proteomics, and has developed a relatively quantitative proteomics method based on trypsin catalysis, which can provide a new means for fluxing comparative analysis of proteins such as disease marker screening, according to a news release on November 11   According to reports, the main contents of proteomics research include protein expression level, post-translational modification, protein-protein interaction, etc The purpose is to master the characteristics and functions of proteins, and ultimately to serve human health On the basis of protein identification, quantitative proteomics technology can detect and analyze the expression level of protein and its existing change forms, so as to find out the differences between normal and abnormal proteins and find markers, so as to realize the early and accurate diagnosis of diseases   One of the breakthroughs of Zou Hanfa's team in the study of new methods of quantitative proteomics is to find that trypsin, which is often used as hydrolase in proteomics, also has the activity of ligase, so that the two-step operation of proteolysis and polypeptide labeling can be completed by using only one enzyme   Compared with the previous quantitative proteomics research using chemical methods, the above enzymatic labeling reaction conditions are mild, with high regional specificity, which greatly reduces the degradation of peptide segments and the occurrence of side reactions, that is to avoid the disadvantages of the previous "chemical reaction" such as harsh conditions, low specificity, chemical reaction interference and so on   Another breakthrough in this study is to label amino acids on peptide segments The new peptide bond formed by this labeling reaction can greatly improve the accuracy of peptide identification Vividly speaking, the previous "task" completed by "trypsin" has been relatively accurate, and "amino acid labeling" has confirmed the "task", the result is undoubtedly more accurate   It is understood that quantitative proteomics has been applied to the study of health problems such as the screening of disease markers and the mechanism of disease formation The new method developed by Dalian Institute of chemistry, Chinese Academy of Sciences is expected to further improve the analytical performance of quantitative proteome and improve the research level in related biomedical fields (end)