Progress has been made in the transformation of glycosyl transferase and biosynthetics of triamcinoids.

Triamcinelgiates are important natural products of plant sources, and their glycosylation increases the diversity of modified structures and functions, thereby increasing their physiological activity, biological utilization, and in-plant cell transport and storage capacity.
Glycosyl transferase is an important catalyst for glycosylation of triamcinoids, and the glycosyl transferase from plant sources is more specialized than that of microbial sources, and the products produced by catalysis are more specialized, and the synthesis of triamcinoids has more physiological functions.
The functional sugar and natural active substances research team of Tianjin Institute of Industrial Biotechnology of the Chinese Academy of Sciences, on the basis of pre-excavation of a number of plant-sourced glyco-based transferases, carried out molecular modification of UCT74AC1 glyco-based transferases and obtained a series of mutants, which improved the catalytic efficiency of different types of triamcinoids by 100 to 10,000 times, and then synthesized a series of new triamcinoidal saccharides using the in-body catalytic function of dominant mutants. In further collaboration with the structural biology and protease research team, the
team carried out the crystal structure analysis and molecular dynamics simulation of the enzyme, revealed the mechanism of action between enzyme molecules and substrates, and provided a theoretical basis for the directional transformation of plant-based glycosyl transferase.
this study proves that plant-sourced glyco-based transferases have important application potential in synthesized natural products.
The research has been supported by the national key research and development project, the National Natural Science Foundation of China, the relevant research results published in the American Chemical Technology Association catalytic journal ACS Catalysis, Tianjin Institute of Technology doctoral student Li Jie and associate researcher Yang Jiangang as co-first author of the paper, researcher Sun Weixia, associate researcher Liu Weidong as side-by-side communication author.
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