Progress has been made in the study of functional dizenose in in-body synthesis

Functional two sugars is a class of low polysaccharides with special effects, consisting of two monosaccharides connected by different glycoside bonds, with high sweetness, low calories, low sugar index characteristics, can be used as probiotics and functional sweeteners in the food field.
addition, certain functional glycosaccharies are widely used in cosmetics, biotechnology and pharmaceuticals because of their unique functions.
preparation method of two sugars is the acidic purification of natural polysaccharides, but the corresponding natural polysaccharide supply is limited, and the acid solution products are complex and the purification cost is high.
with the development of enzyme catalysis technology, people are more and more inclined to look for suitable enzyme catalytic preparation of two sugars.
at present, glycoside phosphatase, which has excellent properties in the synthesis of glycoside bonds, has gradually attracted attention, but its catalytic substrate 1-phosphate is expensive, resulting in a higher cost of synthetic glycosaccharides.
the in-body synthesis biology center research team led by Yu Wei, a researcher at the Tianjin Institute of Industrial Biotechnology of the Chinese Academy of Sciences, designed an in-body polyenzyme catalysis system that converts cheap substrate starch into a variety of functional dizets.
in this system, α-glucan phosphorylase (α-glucan phosphorylase, AlphaGP) and α-glucosinase (α-glucosidase, alphaG) two enzymes work in parallel, converting starch into glucose 1-phosphoric acid and glucose, respectively, followed by glucose 1-phosphate and glucose by different disaccharide phosphorylase (DSP) catalytic synthesis of various functional disgluciates.
The study used dynamic mathematical models to study the reaction process of in-body polyenzyme catalysis system and predict the optimal reaction conditions, and prepared Queanbee iis, fibers, algae sugars, sugars, sugars and other functional two sugars for a single substrate with starch, with yields of more than 80%.
the production mode of functional two sugars in this study has changed the traditional production mode of the existing two sugars, and has greater potential for industrial application.
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