Professor Tian Yun's team at Hunan Agricultural University has made a series of advances in the field of functional lipid green biological manufacturing

Lipid science and health are related to the improvement of the physical fitness of the whole people and the promotion of healthy China's construction, which is a major need of the national strategy to improve the national health level.
peanut tetrylic acid and vernal sorbene are important functional lipids, which are mainly derived from tissue extraction and natural microbial fermentation.
In order to solve the problems of limited resources, long microbial fermentation cycle or low yield in the production process, Professor Tian Yun of the School of Bioscilation of Hunan Agricultural University has made a series of progress in improving the efficient synthesis of peanut tetrylic acid and norophrenic acid through the use of synthetic biotechnology, in cooperation with Nanjing University of Technology, Nanjing Normal University and the National Key Laboratory for the Use of Woody Oil Resources.
degreasing Yeast is an FDA-certified unconventional yeast with unique physiological properties.
Professor Tian Yun's team used the yeast as the chassis cell, by designing and constructing the peanut tyrene acid biosynthesis pathway, analyzing the suitability of different metabolic pathways in the chassis cell, using fusion protein strategy to optimize the target product synthesis method, and finally realized the peanut tyrenic acid in the chassis cell. Efficient Synthesis in Degreasing Yeast Engineering Bacteria (approx. 118.1 mg /L), published in September 2019 in J Agric Food Chem (Chinese Academy of Sciences, Top Journal, IF=4.192) At the same time, the method engineering strategy was used to express HMG1 and DGA1 respectively in the degreasing yeast, optimize the fermentation process of engineering bacteria, and finally realize the engineering bacteria SQ-1 in YPD-80 fermentation medium. The production of 731 mg/L is currently the highest reported using degreased Yeast to synthesize salphore, published in January 2021 in Bioresource Technol (Chinese Academy of Sciences, Top Journal, IF). 7.539), the team, in conjunction with its own research work, reviews the characteristics of the metabolic pathways of the degreasing yeast itself, summarizes the current commonly used strategies for improving the metabolism of target products, and introduces the progress of the production of fatty acids and their derivatives by metabolic engineering, published in Front Microbiol (Chinese Academy of Sciences II, Top Journal, IF-4.236) in January 2021. The
research team will then use systematic metabolic engineering to further transform the chassis cells, strengthen the production performance of engineering bacteria, optimize the fermentation performance of engineering bacteria, improve the ability of grease-degreasing Yeast synthesis of functional lipids, and finally gradually realize the industrialization of functional lipid green biomass manufacturing such as peanut tetrylic acid and sorphate.
The above-mentioned work is mainly by our school's Institute of Biological Science and Technology Dr. Liu Huhu and Tian Yun teachers to guide master's students Tang Wenyan, Wang Dongping and Song Yulan, etc. completed, our school is the first completed unit.
research has been supported by the National Natural Science Foundation of China, the first batch of special funding projects of the China Postdoctoral Science Fund, the Outstanding Youth Project of Hunan Provincial Education Department, the Outstanding Innovation Youth Project of Changsha City and the "Double First Class" construction project of Hunan Agricultural University.
Figure 1: Synthetic biotechnically modified degrease Yeast Synthetic Peanut Tetryene Chart 2: Synthetic Biotechnical Degreased Yeast Synthetic Syndicated Sorphose Figure 3: Degreased Yeast Synthesis Functional Fatty Acids and Their Derivatives Metabolic Path Original Link: