Professor Li Guiying's team and Xing Gengmei's team have made new progress in the field of specific thrombolytic therapy for arterial vein thrombosis

The team of Professor Li Guiying of the Key Laboratory of Molecular Enzymatic Engineering of the Ministry of Education, College of Life Sciences, Jilin University, and the team of Xing Gengmei, Institute of High Energy Physics, Chinese Academy of Sciences, adjusted the particle size of the platelet membrane camouflage delivery system, optimized the permeability and retention of the delivery system in arteriovenous thrombosis, and constructed a physically matched thrombolytic delivery system, which realized the specific thrombolytic treatment
of arterial vein thrombosis 。 The research results, entitled "Clot structure-based physical-matching design of platelet cloaking nano-delivery system facilitates specific arteriovenous thrombolysis", were officially published in Chemical on August 1, 2022 Engineering Journal
。

Thrombosis can induce myocardial infarction, ischemic stroke and pulmonary embolism, posing a great threat
to human health.
However, the clinical thrombolytic drugs have short half-life, poor permeability, uncontrollable bleeding risk and other reasons, which make it difficult for the drug to achieve the expected therapeutic effect
.
At the same time, the physical characteristics such as the composition, structure and shear force of the thrombus site are different, resulting in complex thrombosis treatment strategies and bleeding risks and other clinical treatment problems
.

Based on the differences in the structure and physical characteristics of arteriovenous thrombosis, three nanoparticles of different sizes of platelet membrane camouflaged and carrying the therapeutic drug urokinase were successfully constructed
, and evaluated from the aspects of biological safety, biodistribution and metabolism, the enrichment ability of platelet membrane camouflage nanoparticles in arteriovenous thrombosis, and the effect of arteriovenous thrombolysis in vivo.
The results show that the platelet camouflage nanodelivery system has good biological safety, can prolong the half-life of blood, and effectively reduce the risk of
bleeding.
The thrombotic structure of the arteriovenous veins together with the shear force of the blood flow drives the enrichment ability
of camouflage particles in the thrombus.
And UNP-L and UNP-S showed good long-acting thrombolytic effects
in venous thrombosis models and arterial thrombosis models, respectively.

In this experiment, three platelet membrane-coated nanodelivery systems of different sizes were constructed to physically match the respective characteristics of arteriovenous thrombosis and realize the specific thrombolytic therapy
of nanodrug delivery systems.
This provides a reference
for the design of nanodelivery systems in arterial and intravenous thrombolytic therapy.

Figure 1 Schematic diagram of penetration and enrichment of platelet membrane camouflaged nanoparticles in arteriovenous thrombosis of different sizes

The first author of the research paper is Wang Yujiao, a doctoral student from the College of Life Sciences, Jilin University, Professor Li Guiying from the Key Laboratory of Molecular Enzymatic Engineering of the Ministry of Education, College of Life Sciences, Jilin University, and Xing Gengmei, researcher and assistant researcher Chen Kui of the Institute of High Energy Physics, Chinese Academy of Sciences, are the co-corresponding authors
of the paper.
This research was supported
by the National Natural Science Foundation of China and other projects.

Original link: https://doi.
org/10.
1016/j.
cej.
2022.
135982