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Production of Cybrids in Brassicaceae Species

 Last Update: 2021-03-15
 Source: Internet
 Author: User

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This chapter describes a method of cytoplasm transfer within the brassicaceae family through Ca-PEG-mediated protoplast fusion. The method includes a protocol of nonmutagenic albinism induction based on spectinomycin-induced plastid ribosome deficiency (PRD). The proposed application of spectinomycin-mediated albinism allows speeding up creation of albino lines, as well as of hybrid production with substituted cytoplasm. According to described method cybrids between
Orychophragmus violaceus
and
Brassica napus, O. violaceus
and
Lesquerella fendleri
have been produced. Methods of further molecular analysis are also presented. The time-scale and reliability of described methods are indicated.

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