Principle, application and introduction of components and fluorescent dyes of fluorescence microscope

The principle of fluorescence microscopy is a microscopic observation mode that combines optical microscopy with the emission of fluorescent dyes from compounds through an excitation light source

Fluorescence microscopy is also widely used in the field of histochemistry to detect particles that cannot be seen with conventional microscopy, such as the neurotransmitter amines

It is used in food chemistry to assess the presence, structural organization and spatial distribution of specific food ingredients in products

There is also a fluorescent speckle microscope

Fluorescence microscopy staining is also used in the field of mineralogy

It is also widely used in the textile industry to analyze fiber size

Not only that, the use of fluorescence microscopy can also be used in the field of fluorescent dyes to study ceramic porosity and semiconductor research

Samples suitable for fluorescence microscopy must be fluorescent in order to be suitable for fluorescence microscopy, and common methods for fluorescence sample preparation include labeling with fluorescent dyes, expressing fluorescent proteins, or exploiting the autofluorescence of the sample
.

Some stains in fluorescence microscopy are available for a variety of biological substances
.
Some small molecules have their own fluorescence and preferred biomolecules to which they bind
.
Examples include DAPI and Hoechst, which are excited by UV light; and DRAQ5 and DRAQ7, which are excited best by red light
.
Each of them binds to the minor groove of DNA and in this way marks the nucleus
.
Some other stains are peptides, toxins and drugs and bind to cellular structures
.
These must be derived with fluorescent reporters for this to work
.

The binding of antibodies to their corresponding antigens, thereby labeling proteins or other molecules in cells, is achieved through the process of immunofluorescence
.
Modern genetic knowledge and technology allow scientists to edit DNA
.
The same technique can be used to genetically modify proteins to add fluorescent protein reporter genes, which means scientists can make a certain protein fluoresce
.
Once it fluoresces, it can be tracked directly, even in living cells
.


Suzhou Alpha Biological Experiment Equipment Co.
, Ltd.
has been focusing on the supply of biological laboratory equipment and reagent consumables for 13 years.
The instruments and equipment include fluorescence microscopes, inverted microscopes, biological microscopes, portable microscopes, etc.
At the same time, the supply of cell biology reagents and consumables mainly includes cell culture.
Reagents, culture medium, PBS buffer, culture flasks, cell shake flasks, cell transfection reagents, fluorescent dyes, LIP2000, LIP3000,
etc.