PLoS patch: how Crohn's disease-related bacteria produce antibiotic tolerance

November 18, 2019 / biool / -- according to a recent study published in the journal PLoS patterns, bacteria associated with Crohn's disease rely on a variety of stress responses to survive, reproduce and produce tolerance in macrophages within leukocytes Crohn's disease is a chronic disease that causes inflammation and irritation of the digestive tract The disease is characterized by an imbalance in the intestinal microbiome In particular, adherence to invasive Escherichia coli (AIEC) strains is associated with the disease AIEC can settle in intestinal cells, survive and replicate in macrophages In this new study, the researchers demonstrated that the AIEC lf82 strain can switch between "replication state" and "non replication state" in macrophages This transformation may be caused by the "stress response" immediately after the macrophage is infected, or it may be caused by DNA damage and SOS stress response during replication in the macrophage in the later stage (image source: www Pixabay Com) first, the "early stress response" was able to suspend the replication activity of AIEC lf82 for several hours, during which a large number of AIEC lf82 obtained the ability to tolerate antibiotic treatment Later, when the replication of AIEC started again, it needed to repair DNA, indicating that AIEC had accumulated DNA damage in the first few hours after infection In the second stage, the AIEC lf82 population in the growing state often had inactive bacteria, which increased the proportion of antibiotic resistant AIEC lf82 by as much as 10% The results showed that these bacteria pools with antibiotic tolerance and no "division" may be the cause of the residual bacteria in the host and the recurrence of infection Sources of information: how Crohn's disease associated bacteria tolerance antiotics original sources: GA ë LLE demarre, Victoria student, Hanna Schenk, Emilie Rousseau, Marie AGN è s bringer, Nicolas barnech, guy Tran Van nhieu, Sylvie Rimsky, Silvia de Monte, Olivier Espéli The Crohn’s disease-associated Escherichia coli strain LF82 relies on SOS and stringent responses to survive, multiply and tolerate antibiotics within macrophages PLOS Pathogens , 2019; 15 (11): e1008123 DOI: 10.1371/journal.ppat.1008123