-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Image: The Illinois Advanced Biofabrication Biofactory (iBioFAB) supports the automated construction of plasmids through a new platform called PlasmidMak.
Image Courtesy: Center for Advanced Bioenergy and Bioproducts Innovation
Plasmids are one of the most basic tools of recombinant DNA technolo.
Researchers have developed several strategies to address this limitation by automating the construction of plasmid D.
To address this, Behnam Enghiad, Xue Pu, and other researchers at the Center for Advanced Bioenergy and Bioproduct Innovation (CABBI) at the University of Illinois at Urbana-Champaign have developed a versatile, automated plasmid design and construction platform, saying for PlasmidMak.
Creating plasmids starts with desi.
Once the plasmid design is complete, it will be submitted to the plasmid manufacturing team, and the order for the plasmid will go to the Illinois Bio Advanced Biofabrication Facility (iBioFAB) for producti.
The plasmid construction process is automated: samples are prepared by polymerase chain reaction (PCR), purified, transformed by DNA sequence assembly, and frozen for plasmid confirmation with little human involveme.
In addition to the automation and precision provided by iBioFAB, the PlasmidMaker platform pioneers a new highly flexible method for assembling multiple DNA fragments into plasmids using PfAgo-based artificial restriction enzymes (ARE.
Restriction enzymes have long been used in plasmid construction because they can cut DNA molecules at specific base sequences, called recognition sequenc.
"In previous DNA assembly methods, it was often difficult to find suitable restriction enzymes to cut plasmids and replace DNA fragments," said Huimin Zhao, one of the authors and the Steven.
As one of four DOE-funded bioenergy research centers, CABBI team members hope PlasmidMaker will accelerate the development of synthetic biology for biotechnology applicatio.
"This tool will be made available to researchers at CABBI, and we hope to eventually make it available to all researchers at the other three bioenergy research centers," Zhao sa.
