-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
-
Cosmetic Ingredient
- Water Treatment Chemical
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
The transgenic
RescueMu
lines were designed for and successfully used in our maize gene discovery project. The pBluescript-containing
RescueMu
transposon can be readily recovered by a procedure called plasmid rescue. Plasmid rescue is a technique for recovering bacterial plasmids from transgenic eukaryotic genomic
DNA
. Total maize DNA was first digested with restriction enzyme(s), ligated, and then transformed into
E. coli
cells. Colonies were recovered under selection against the antibiotic marker(s) in the transgene vector. Ampicillin or carbenicillin was used for
RescueMu
transgene recovery. The flanking genomic sequences at
RescueMu
insertion sites were simultaneously captured and then sequenced using
RescueMu
-readout primers. Genomic DNA from an individual plant or from pooled samples of up to ∼50 plants could be used in a single rescue. Because the majority of transgenic constructs currently used in flowering plants were made in the form of plasmids, this protocol could therefore be adapted by and useful to researchers involved in other transgenic work and be versatile for characterizing transgene loci.
