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Biologists often use green fluorescent protein (GFP) to see what's happening
inside cells.
For the first time, scientists isolated GFP from jellyfish, a protein that transforms light from one color to another
.
By connecting it to other proteins, researchers can know if cells produce those proteins and where they
can be found within the cell.
This in turn illustrates how cells transmit and use genes
.
The problem is that this often requires expensive equipment, such as fluorescence microscopy, and can be time-consuming
.
In this study, the researchers describe how a particular type of green fluorescent protein "observes" the production
of the protein with the naked eye.
By modifying the plant's genes, the team could see that long-wave ultraviolet (UV) light is provided with simple black light to produce green fluorescent proteins
.
inside cells.
For the first time, scientists isolated GFP from jellyfish, a protein that transforms light from one color to another
.
By connecting it to other proteins, researchers can know if cells produce those proteins and where they
can be found within the cell.
This in turn illustrates how cells transmit and use genes
.
The problem is that this often requires expensive equipment, such as fluorescence microscopy, and can be time-consuming
.
In this study, the researchers describe how a particular type of green fluorescent protein "observes" the production
of the protein with the naked eye.
By modifying the plant's genes, the team could see that long-wave ultraviolet (UV) light is provided with simple black light to produce green fluorescent proteins
.
This study demonstrates real-time imaging
of cell and molecular events in a large number of plants with the naked eye and a black flashlight.
This will provide rapid and affordable screening
for research and development or real-time monitoring of molecular events in mature plants.
Reporter genes are attached to other genes of interest, providing an inexpensive, rapid, and sensitive assay
for studying gene delivery and gene expression.
These reporters have long been an essential tool
for live-cell imaging.
Today, imaging and analysis have become easier
with the development of UV-visible fluorescence reporters.
Scientists at Oak Ridge National Laboratory conducted the study with the goal of improving the use and efficiency
of these reporter genes in two herbaceous plants (Arabidopsis and tobacco) and two woody plants (poplar and citrus).
After designing and constructing a GFP ultraviolet reporter protein (eYGFPuv) that provides enhanced signals to all plant species tested, the researchers demonstrated that strong fluorescence can be captured using fluorescence
microscopy or ultraviolet light.
In addition, this excitation of ultraviolet reporter factors can be observed at a wide range of scales from seedlings under one meter to whole plants, without the need for special radiation filters
.
For example, by using a simple UV flashlight, scientists demonstrated how this new reporter can facilitate rapid quantification
of the transformation efficiency of plant systems.
These improved features will make this newly developed GFP-UV reporter a valuable tool
for widespread use in plant science research.
