Pharmaceutical grade glycerin 500 grams a bottle of small trial research and development of preparation excipients

Pharmaceutical grade glycerin 500 grams a bottle of small test research and development of preparation excipients [check] pH Take this product 25.
0g, add water to dilute into 50ml, mix well, add phenolphthalein indicator liquid 0.
5ml, the solution should be colorless, add 0.
1mol/L sodium hydroxide solution 0.
2ml, the solution should appear pink
.

Color Take 50ml of this product, put in a 50ml Natkovicin cuvette tube, compare with the control solution (take the colorimetric potassium dichromate solution 0.
2ml, add water to dilute to 50ml), not deeper
.

Chloride Take 5.
0g of this product, check according to law (General 0801), and compare with the control solution made of 5.
0ml of standard sodium chloride solution, it shall not be more concentrated (0.
001%)
.

Sulfate Take 10.
0g of this product, check according to law (General 0802), and compare with the control solution made of 2.
0ml of standard potassium sulfate solution, it shall not be more concentrated (0.
002%)
.

Aldehyde and reducing substances Take 1.
0g of this product, put it in a 50ml measuring bottle, add 25ml of water to dissolve, add 10% hydrochloric acid and thiazolidone hydrazone solution (adjust the pH value to 4.
0 with 0.
02mol
/L sodium hydroxide solution.

2ml of fresh) 2ml, let stand for 30 minutes, add 5ml of newly prepared 0.
5% ferric chloride solution, shake well, let stand for 5 minutes, add methanol to dilute to the scale, shake well
.

According to the ultraviolet-visible spectrophotometry (General Rule 0401), the absorbance is determined at a wavelength of 655 nm, and the absorbance of the test solution shall not be greater than the absorbance of the control solution [per 1 ml containing formaldehyde (CH2O) 5.
0 μg] 2.
0 ml after the same method treatment
.

Sugar Take 5.
0g of this product, add 5ml of water, mix well, add dilute sulfuric acid 1ml, heat on the water bath for 5 minutes, add 2mol/L sodium hydroxide solution without carbonate (take the appropriate amount of sodium hydroxide, add water to shake to dissolve into a saturated solution, after cooling, place in a polyethylene plastic bottle, and let it stand tightly for a few days, Take 5.
6ml of the supernatant, add a new boiling and put cold water into 50ml, shake well, that is, to get) 3ml, drop add copper sulfate test solution 1ml, mix well, should be a blue clear solution, continue to heat on the water bath for 5 minutes, the solution should still be blue, no precipitation generated
.

Fatty acids and lipids Take 40.
0g of this product, add a new boiling and put cold water 40ml, then carefully add sodium hydroxide titration solution (0.
1mol/L) 10ml, shake well, boil for 5 minutes, let cool, add a few drops of phenolphthalein indicator solution, titrate with hydrochloric acid titration solution (0.
1mol/L) until the red disappears, and correct
the titration results with blank tests.

The consumed sodium hydroxide titration solution (0.
1 mol/L) shall not exceed 4.
0 ml
.

Easy charcoal Take 4.
0g of this product, add 5ml of sulfuric acid drop-by-drop under shaking, the control temperature during the process shall not exceed 20 ° C, after standing for 1 hour, such as color development, compared with the same volume of control solution (take colorimetric cobalt chloride solution 0.
2ml, colorimetric potassium dichromate solution 1.
6ml and water 8.
2ml), not deeper
.

Chloride Take 5.
0g of this product, add 10ml of water and 2mol/L sodium hydroxide solution 1ml, mix well, add nickel aluminum alloy 50mg, heat on the water bath for 10 minutes, cool to room temperature, filter, wash the container and filter residue in 20ml with water, collect the filtrate and wash into a 50ml Mnvish colorimeter, add nitric acid 0.
5ml, mix well, then add silver nitrate test solution 0.
5ml, add water to the scale, shake well
.

Do not go deeper (0.
003%)
compared to the control solution made of 15 ml of standard sodium chloride solution.