 Home > Biochemistry News > Plant Extracts News > PCR and RFLP Analyses Based on the Ribosomal Protein Operon

PCR and RFLP Analyses Based on the Ribosomal Protein Operon

 Last Update: 2021-03-13
 Source: Internet
 Author: User

Tags

anticoagulant drugs classification

omeprazole classification

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Differentiation and classification of phytoplasmas have been primarily based on the highly conserved 16S rRNA gene. RFLP analysis of 16S rRNA gene sequences has identified 31 16S rRNA (16Sr) groups and more than 100 16Sr subgroups. Classification of phytoplasma strains can, however, become more refined and specific if moderately conserved genes, such as the ribosomal protein (
rp
) genes, are used as genetic markers. The use of additional genetic markers enhances the resolving power of phytoplasma classification. This chapter describes the methodology of detection, differentiation, and classification of phytoplasma strains based on
rp
gene sequences. RFLP analysis of amplicons obtained by group- or subgroup-specific
rp
gene-based primers is used for finer differentiation of phytoplasma strains within a given group or subgroup. The
rp
gene-based classification not only readily resolves 16Sr subgroups within a given 16Sr group, but also provides finer differentiation of closely related phytoplasma strains. Many individual 16Sr subgroups can be further differentiated into two or more distinct
rp
subgroups.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Ginsenoside-Rg5

CAS No.: 186763-78-0

Industrial grade

$1/KG EXW

3-O-Ethyl-L-ascorbic acid /86404-04-8

CAS No.: 86404-04-8

pharmaceutical Grade

$10/KG FOB

TETRAHYDROPIPERINE / 23434-88-0

CAS No.: 23434-88-0

pharmaceutical Grade

$10/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.