PCE Jiangsu Academy of Agricultural Sciences Zhou Tong's team and Cornell University Huajian team cooperated to discover the transcriptional regulation mechanism of plant intracellular immune receptor genes

Plant intracellular immune receptor NLR genes are highly regulated at the transcriptional and post-transcriptional levels to balance plant defense and growth
.

At present, the regulation of NLR protein has been deeply studied.
However, since NLR genes are often located in gene clusters and are regulated by large regions of chromatin, transgenic methods cannot be used to study their cis-elements.
The regulatory mechanisms of induction, repression or co-expression are poorly understood
.

Recently, researcher Zhou Tong's research group from the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences and Professor Hua Jian's research group from Cornell University published a paper entitled "In situ deletions reveal regulatory components for expression of an intracellular immune receptor gene" on Plant, Cell & Environment.
and its co-expressed genes in Arabidopsis” research paper, discovered multiple cis factors and two trans transcription factors that regulate the transcriptional regulation of the immune receptor gene SNC1 in plant cells, and initially revealed its regulatory mechanism
.

To search for cis-regulatory elements of SNC1, the researchers created a series of deletions at the SNC1 site through a dual-targeted CRISPR/Cas9 system, spanning a region 2 kb upstream of the translation initiation site and 4 kb downstream of the stop codon
.

Through the analysis of 10 deletion mutants, multiple transcription-promoting and repressing cis-elements were identified, such as upstream region C2, C5 and downstream region C9 are positive cis-elements, upstream region C8 and downstream region C10 are negative cis-elements type components (Figure 1)
.

Transcription factors LHY1 and bHLH28, which may be bound to two transcription promoting elements, were further identified and their functions in the regulation of SNC1 gene expression were verified (Fig.
2)
.

These results provide new clues for the transcriptional regulation mechanism of the plant intracellular immune receptor gene SNC1
.

Fig.
1 The putative cis-acting elements and transcription factors at the SNC1 locusFig.
2 The roles of LHY1 and bHLH28 in the regulation of SNC1 expression He is the co-first author of the paper, researcher Zhou Tong of Jiangsu Academy of Agricultural Sciences and Professor Hua Jian of Cornell University are co-corresponding authors
.

The research was supported by the National Natural Science Foundation of China (31761143012), the National Natural Science Foundation of the United States (IOS-1946174), and the Jiangsu Provincial Postdoctoral Research Fund
.

Link to the original text: https://doi.
org/10.
1111/pce.
14293 This article is reproduced from the Plant and Environment PCE Plant Biotechnology Pbj Exchange Group In order to more effectively help scientific researchers obtain relevant information, Plant Biotechnology Pbj has established a special Wechat group, Plant Biotechnology Journal submission and literature-related questions, public account content and public account submission questions will be answered in the group, and at the same time, academic exchanges and collision of ideas are encouraged in the group
.

In order to ensure a good discussion environment in the group, please add Xiaobian WeChat first, scan the QR code to add, and then we will invite you to join the group in time
.

Tips: When adding Xiaobian WeChat and after joining the group, please be sure to note the school or unit + name, PI at the end, and we will invite you to join the PI group
.