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Optimization of Steady-State 13C-Labeling Experiments for Metabolic Flux Analysis

 Last Update: 2021-01-17
 Source: Internet
 Author: User

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While steady-state
13
C metabolic flux analysis is a powerful method for deducing multiple fluxes in the central metabolic network of heterotrophic and mixotrophic plant tissues, it is also time-consuming and technically challenging. Key steps in the design and interpretation of steady-state
13
C labeling experiments are illustrated with a generic protocol based on applications to plant cell suspension cultures.

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