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The Wnt signaling pathway plays a very important role in the growth and development of stem cells, maintenance of stemness, tumorigenesis and metastasis
2021 Nian 10 Yue 12 days, I was in the hospital laboratory Eastern Nature Communications , published online entitled "Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division" of research papers
SET-seq first isolation of nuclei of the same cell and cytoplasmic RNA , then based on Tn5 transposase, the nucleus of the transfected protein bound chromatin fragments be apparent library construction ; while inverting synthesized cytoplasmic RNA of the cDNA , using Tn5 transfer The enzyme constructs a transcriptome library on the cDNA/RNA hybrid strand (Figure 1A )
Researcher Fang Dong is the corresponding author of this article.
FIG 1 through S the ET - SEQ confirmed that in the Wnt .
Original link:
https://doi.
references:
1Habib, SJet al.
2Ma, B.
